PXD044226

PXD044226 is an original dataset announced via ProteomeXchange.

Title	Investigating the effect of fluid flow on glomerular cell-derived matrix
Description	The glomerular basement membrane has a complex composition and structure in vivo. In vitro models of the glomerular capillary wall have been developed using microfluidic and perfusion systems, however, how the extracellular matrix alters under static and flow conditions has not yet been investigated. Here we performed proteomic analyses following a previously described workflow (Byron et al., 2014; Rashid et al., 2012; Soteriou et al., 2013) to investigate the protein composition of podocyte and glomerular endothelial coculture cell-derived matrix and whether this was altered when cells experienced shear stress induced by flow. Our results reveal a distinct separation between flow and static-treated samples and highlight compositional changes in the matrix in the microfluidic in vitro model, and confirm the flow impacted the abundance of certain matrix proteins.
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-02_06:29:36.055.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044226
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mychel Morais
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-31 07:51:14	ID requested
⏵ 1	2026-02-02 06:29:36	announced

10.1016/J.CDEV.2024.203923;

10.6019/PXD044226;

submitter keyword: Glomerular matrix, Microfluidic bioreactor,Glomerular filtration barrier

Rachel Lennon
contact affiliation	Director of the Wellcome Centre for Cell-Matrix Research Wellcome Trust Centre for Cell Matrix Research School of Biological Sciences Faculty of Biology, Medical and Health Sciences University of Manchester
contact email	rachel.lennon@manchester.ac.uk
lab head
Mychel Morais
contact affiliation	The University of Manchester
contact email	mychel.morais@manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044226
     1. Label: PRIDE project
     2. Name: Investigating the effect of fluid flow on glomerular cell-derived matrix