PXD044215
PXD044215 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The potential of plasma HLA peptides in diagnostic and therapy beyond neoepitopes |
| Description | Distinction of non-self from self is the major task of the immune system. Immunopeptidomics studies the peptide repertoire presented by the human leukocyte antigen (HLA) protein, usually on tissues. However, HLA peptides are also bound to plasma soluble HLA (sHLA), but little is known about their origin and potential for biomarker discovery in this readily available biofluid. Currently, immunopeptidomics is hampered by complex workflows and limited sensitivity, generally requiring several mL of plasma for the detection of hundreds of HLA peptides. Here, we take advantage of recent improvements in the throughput and sensitivity of mass spectrometry (MS)-based proteomics to develop a highly-sensitive, automated and economical workflow for HLA peptide analysis, termed Immunopeptidomics by Biotinylated Antibodies and Streptavidin (IMBAS). IMBAS-MS quantifies more than 5,000 HLA class I peptides from only 200 uL of plasma, in just 30 minutes. Our technology revealed that the plasma immunopeptidome of healthy donors is remarkably stable throughout a year and strongly correlated between individuals with overlapping HLA types. Immunopeptides originating from diverse tissues, including the brain, are proportionately represented. We conclude that sHLAs are a promising avenue for immunology and precision oncology. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-12-05 |
| AnnouncementXML | Submission_2023-12-05_04:01:12.031.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | mario oroshi |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Cysteinyl |
| Instrument | timsTOF SCP; instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-07-31 02:39:19 | ID requested | |
| ⏵ 1 | 2023-12-05 04:01:12 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: sHLA, immunopeptidomics , plasma immunopeptidome, IMBAS-MS |
Contact List
| Matthias Mann | |
|---|---|
| contact affiliation | Proteomics and Signal Transduction Max Planck Institute of Biochemistry Am Klopferspitz 18 D-82152 Martinsried |
| contact email | mmann@biochem.mpg.de |
| lab head | |
| mario oroshi | |
| contact affiliation | MPI |
| contact email | oroshi@biochem.mpg.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092557/ |
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