PXD044214

PXD044214 is an original dataset announced via ProteomeXchange.

Title	Direct binding of type III secretion effectors to cytosolic sorting platform complexes in live bacteria indicates an effector shuttling mechanism
Description	The bacterial type III secretion system, also called injectisome, translocates effector proteins into eukaryotic target cells through a hollow needle that is anchored in the bacterial membranes. The recruitment of effector proteins to the machinery and the resulting export hierarchy involve the sorting platform, a conserved set of interacting proteins at the cytosolic interface of the injectisome. To gain insight into these processes, we localized and measured the movement of different functional fluorescently labeled sorting platform components in live Yersinia enterocolitica. The sorting platform proteins were part of ~5.1 and ~17.9 injectisomes per bacterium under non-secreting and secreting conditions, respectively. However, they also displayed a considerable mobile cytosolic pool. Using single particle tracking photoactivated localization microscopy, we were able to show the interaction of different components of the sorting platform with effector proteins in the cytosol of live bacteria. We found that effector proteins directly bind to the sorting platform proteins SctQ and SctL. In wild-type bacteria these proteins form larger cytosolic protein complexes involving the ATPase SctN and the membrane connector SctK. The mobility and composition of these complexes is modulated in presence of effector proteins and their chaperones, and upon initiation of secretion, which additionally leads to the emergence of large soluble complexes. Our quantitative data supports an effector shuttling mechanism, in which sorting platform protein bind to effectors in the cytosol and deliver the cargo to the export gate at the membrane-bound injectisome.
HostingRepository	PRIDE
AnnounceDate	2023-10-10
AnnouncementXML	Submission_2023-10-10_07:32:27.689.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TimoGlatter
SpeciesList	scientific name: Yersinia enterocolitica subsp. enterocolitica NBRC 105693; NCBI TaxID: 1218100;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-31 02:32:02	ID requested
⏵ 1	2023-10-10 07:32:28	announced

Dataset with its publication pending

submitter keyword: Type 3 secretion

TimoGlatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Karl-von-Frisch-Str. 10 35043 Marburg Germany
contact email	timo.glatter@mpi-marburg.mpg.de
lab head
TimoGlatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Marburg
contact email	timo.glatter@mpi-marburg.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044214
     1. Label: PRIDE project
     2. Name: Direct binding of type III secretion effectors to cytosolic sorting platform complexes in live bacteria indicates an effector shuttling mechanism