PXD044209

PXD044209 is an original dataset announced via ProteomeXchange.

Title	EPURISp: Combining Enzymatic Digestion, Ultrafiltration, and Rapid In-situ Sample Purification for High-performance Proteomics
Description	High-performance liquid tandem mass spectrometry (HPLC-MS) is widely employed for protein analysis in biological systems. However, conventional proteomic sample pre-treatment methods suffer from multiple steps and poor reproducibility. In this study, we introduce EPURISp (Enzymatic Digestion with Ultrafiltration and Rapid In-situ Sample Purification), a novel proteomic pre-treatment technique that combines enzymatic digestion, ultrafiltration, and one-step temperature-controlled vacuum drying for efficient desalting. The EPURISp method exhibits excellent protein recovery rates across a wide range of molecular weights and hydrophilicity, surpassing traditional C18 desalting approaches. Practical proteomic analysis utilizing EPURISp demonstrates the highest protein identification yield with remarkable reproducibility, particularly advantageous in membrane protein identification. Notably, EPURISp exhibits superior performance in minimizing oxidation and deamidation modifications compared to conventional FASP methods. This innovative EPURISp method represents a significant advancement in proteomics analysis, providing reliable and efficient results for mass spectrometry.
HostingRepository	iProX
AnnounceDate	2023-07-29
AnnouncementXML	Submission_2024-02-29_18:17:48.464.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuemeng Ji
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-30 19:34:34	ID requested
1	2023-09-04 20:47:42	announced
⏵ 2	2024-02-29 18:17:49	announced	2024-03-01: Update information.

Lu P, Shan M, Ji X, Deng F, Wang Y, EPURISp: Combining Enzymatic Digestion, Ultrafiltration, and Rapid In Situ Sample Purification for High-performance Proteomics. J Proteome Res, 22(10):3392-3400(2023) [pubmed]

submitter keyword: EPURISp, Ultrafiltration, Proteomics, desalting

Xuemeng Ji
contact affiliation	Nankai Univeristy
contact email	jixuemeng@nankai.edu.cn
lab head
Xuemeng Ji
contact affiliation	Nankai Univeristy
contact email	jixuemeng@nankai.edu.cn
dataset submitter

iProX dataset URI