PXD044197

PXD044197 is an original dataset announced via ProteomeXchange.

Title	Mapping three-dimensional intratumor proteomic heterogeneity in uterine serous carcinoma by multiregion microsampling
Description	Uterine serous carcinoma (USC) represents only a small proportion of all uterine cancer cases, but patients with this aggressive subtype typically have high rates of chemotherapy resistance, disease recurrence, and constitute a disproportionately high percentage of the deaths. Improving the clinical management of USC is predicated by better characterization of the tumor microenvironment (TME) and the molecular features driving disease pathology. To improve our understanding of intratumoral heterogeneity (ITH) within the USC TME, we investigated proteome and transcriptome alterations in spatially resolved laser microdissection (LMD) enriched cellular subpopulations from nine USC patient tumor tissue specimens. LMD enriched samples were analyzed via liquid chromatography-tandem mass spectrometry (LC-MS/MS), reverse phase protein microarray (RPPA), and targeted RNA-sequencing (RNA-seq).
HostingRepository	PRIDE
AnnounceDate	2024-01-24
AnnouncementXML	Submission_2024-01-24_12:29:59.584.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Conrads
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-28 23:12:27	ID requested
⏵ 1	2024-01-24 12:29:59	announced

10.1186/S12014-024-09451-2;

submitter keyword: laser microdissection, proteomics, proteogenomics, intratumor heterogeneity,uterine serous carcinoma, spatial proteomics, tumor microenvironment

Thomas P.
contact affiliation	Director of Women’s Health Research Women’s Service Line Inova Health System
contact email	conrads@whirc.org
lab head
Thomas Conrads
contact affiliation	Inova Health System
contact email	conrads@whirc.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD044197

PRIDE project URI

• PRIDE
  1. PXD044197
     1. Label: PRIDE project
     2. Name: Mapping three-dimensional intratumor proteomic heterogeneity in uterine serous carcinoma by multiregion microsampling