PXD044186

PXD044186 is an original dataset announced via ProteomeXchange.

Title	Ciliopathy-associated missense mutations in IFT140 are hypomorphic and have edgetic effects on protein interaction networks
Description	The mechanisms underlying recessive Mendelian diseases and the interplay between genotype and phenotype still need to be better understood. It is therefore necessary to characterise the functional effects of missense mutations at the protein level. Here we focus on missense mutations in the intraflagellar transport protein IFT140, which forms part of the IFT complex A (IFT-A), a crucial component of the ciliary machinery. Mutations in IFT140 can cause a vast spectrum of diseases belonging to the group of ciliopathies, reaching from isolated retinal dystrophy to severe skeletal abnormalities and multi-organ diseases such as Mainzer-Saldino and Jeune syndrome. We hypothesise that missense mutations in IFT140 are hypomorphic leading to quantitative effects on a subset of protein-protein interactions. This may affect complex stability as well as perturbations of protein interaction networks. In this work we assessed how 24 missense mutations in IFT140 affect interactions with other IFT and effector proteins using affinity purification coupled to mass spectrometry. Our data reveals that several mutations in IFT140 are hypomorphic and disrupt the stability of the IFT-A complex to varying degrees in a quantitative way. Allelic combination and the degree of IFT-A complex disruption in analysed missense mutations correlates with the severity of the observed phenotype in a subset of patients. In addition, we show that a distinct subset of mutations in IFT140 shows edgetic effects by disrupting specific PPIs rather than causing a total loss of IFT-A binding. This is the case e.g. with the disease-associated protein TULP3 which is involved in cilia-dependent sonic hedgehog signalling.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_11:49:52.097.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tina Beyer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-28 08:42:33	ID requested
⏵ 1	2025-05-06 11:49:53	announced

10.1016/j.mcpro.2025.100916;

Beyer T, Diwan GD, Leonhard T, Dahlke K, Klose F, Stehle IF, Seda M, Bolz S, Woerz F, Russell RB, Jenkins D, Ueffing M, Boldt K, Ciliopathy-Associated Missense Mutations in IFT140 are Tolerated by the Inherent Resilience of the IFT Machinery. Mol Cell Proteomics, 24(3):100916(2025) [pubmed]

submitter keyword: cilia,Intraflagellar transport, protein interaction, ciliopathy, hypomorph, missense mutation, edgetic

Dr. Karsten Boldt
contact affiliation	Institute for Ophthalmic Research University of Tübingen
contact email	karsten.boldt@uni-tuebingen.de
lab head
Tina Beyer
contact affiliation	Uniklinik Tübingen
contact email	tina.beyer@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044186
     1. Label: PRIDE project
     2. Name: Ciliopathy-associated missense mutations in IFT140 are hypomorphic and have edgetic effects on protein interaction networks