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PXD044185

PXD044185 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSleeve-Gastrectomy results in improved metabolism and a massive stress response of the liver proteome in a mouse model of nonalcoholic steatohepatitis
DescriptionIntroduction: Bariatric surgery has been shown to improve the histopathological findings in patients with obesity and non-alcoholic steatohepatitis, but there are also reports about non-responder or progressive disease after bariatric interventions. Therefore, it is of utmost importance to understand the pathophysiological processes in the liver after bariatric surgery. Material and Methods: In the present study, mice were fed a Western Diet to induce non-alcoholic steatohepatitis and sleeve-gastrectomy (SG), or sham operation was performed. Mice were observed for two or eight weeks after surgery and metabolic assessment was performed throughout the experiment. Histopathology, fluorescence associated cell sorting and proteomic analysis were conducted to evaluate hepatic inflammation, liver metabolism and affected signaling pathways. Results: Weight loss was higher, and metabolism significantly improved after SG. Two weeks after SG major inflammatory and regulatory disturbances in the liver were observed, but these disturbances decreased in the long-term. Conclusion: The rapid weight loss and decrease of hepatic fat after SG lead to a proinflammatory response in the liver in the early phase after surgery, which changes to a more moderate immune response in the long-term. We suggest a preoperative risk stratification and postoperative surveillance depending on the histopathological findings.
HostingRepositoryPRIDE
AnnounceDate2024-10-21
AnnouncementXMLSubmission_2024-10-21_02:33:51.467.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristian Preisinger
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-28 08:32:54ID requested
12024-10-21 02:33:51announced
Publication List
10.1016/J.HELIYON.2024.E38678;
Keyword List
submitter keyword: liver, NASHH, LC-MS, mouse
Contact List
Andreas Kroh
contact affiliationDepartment of General, Visceral and Transplantation Surgery, RWTH Aachen University Hospital, Aachen, Germany
contact emailakroh@ukaachen.de
lab head
Christian Preisinger
contact affiliationProteomics Facility, IZKF Aachen, University Clinic RWTH Aachen
contact emailcpreisinger@ukaachen.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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