PXD044185

PXD044185 is an original dataset announced via ProteomeXchange.

Title	Sleeve-Gastrectomy results in improved metabolism and a massive stress response of the liver proteome in a mouse model of nonalcoholic steatohepatitis
Description	Introduction: Bariatric surgery has been shown to improve the histopathological findings in patients with obesity and non-alcoholic steatohepatitis, but there are also reports about non-responder or progressive disease after bariatric interventions. Therefore, it is of utmost importance to understand the pathophysiological processes in the liver after bariatric surgery. Material and Methods: In the present study, mice were fed a Western Diet to induce non-alcoholic steatohepatitis and sleeve-gastrectomy (SG), or sham operation was performed. Mice were observed for two or eight weeks after surgery and metabolic assessment was performed throughout the experiment. Histopathology, fluorescence associated cell sorting and proteomic analysis were conducted to evaluate hepatic inflammation, liver metabolism and affected signaling pathways. Results: Weight loss was higher, and metabolism significantly improved after SG. Two weeks after SG major inflammatory and regulatory disturbances in the liver were observed, but these disturbances decreased in the long-term. Conclusion: The rapid weight loss and decrease of hepatic fat after SG lead to a proinflammatory response in the liver in the early phase after surgery, which changes to a more moderate immune response in the long-term. We suggest a preoperative risk stratification and postoperative surveillance depending on the histopathological findings.
HostingRepository	PRIDE
AnnounceDate	2024-10-21
AnnouncementXML	Submission_2024-10-21_02:33:51.467.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Preisinger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-28 08:32:54	ID requested
⏵ 1	2024-10-21 02:33:51	announced

10.1016/J.HELIYON.2024.E38678;

submitter keyword: liver, NASHH, LC-MS, mouse

Andreas Kroh
contact affiliation	Department of General, Visceral and Transplantation Surgery, RWTH Aachen University Hospital, Aachen, Germany
contact email	akroh@ukaachen.de
lab head
Christian Preisinger
contact affiliation	Proteomics Facility, IZKF Aachen, University Clinic RWTH Aachen
contact email	cpreisinger@ukaachen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044185
     1. Label: PRIDE project
     2. Name: Sleeve-Gastrectomy results in improved metabolism and a massive stress response of the liver proteome in a mouse model of nonalcoholic steatohepatitis