PXD044168 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative proteomic analysis of eggs and ovarian fluid in Siberian sturgeon: Insights into composition and functional significance |
Description | Sturgeon species, considered living fossils, exhibit unique reproductive characteristics, making it crucial to comprehend the molecular processes governing the formation and quality of their eggs. However, there is a notable lack of comprehensive data concerning the protein composition analysis of sturgeon eggs and ovarian fluid (OF) and their functional significance. To address this knowledge gap, this study aimed to conduct a comprehensive comparative proteomic analysis of Siberian sturgeon eggs and OF using liquid chromatography-mass spectrometry (LC-MS/MS). A total of 566 proteins were identified in eggs, while 617 proteins were identified in OF, with 772 proteins showing differential abundance. Among the differentially abundant proteins, 407 were more abundant in eggs, while 365 showed higher abundance in OF. Furthermore, we identified 288 proteins specific to eggs and 339 proteins specific to OF, along with the top ten most abundant proteins in each. Functional annotation analysis unveiled enriched metabolic pathways, such as oxidative phosphorylation and fatty acid metabolism, as well as protein ubiquitination and translation, in eggs. Conversely, ovarian fluid proteins primarily associated with immune system processes, including the complement and coagulation cascade, neutrophil and leukocyte-mediated immunity, cholesterol metabolism, and regulation of actin cytoskeleton. This study presents the first comprehensive characterization of the protein composition of sturgeon ovarian fluid and eggs, shedding light on their distinct functional roles. The findings not only advance our understanding of sturgeon reproduction but also shed light on egg-OF signaling and origin of the OF proteins. Moreover the identified proteins offer potential biomarkers for predicting egg quality contributing to the development of effective breeding strategies for sturgeon species. |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-18 |
AnnouncementXML | Submission_2023-12-18_06:24:00.456.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD044168 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Agata Malinowska |
SpeciesList | scientific name: Acipenser baerii; NCBI TaxID: 27689; |
ModificationList | methylthiolated residue; monohydroxylated residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-07-28 02:42:24 | ID requested | |
⏵ 1 | 2023-12-18 06:24:00 | announced | |
Publication List
Keyword List
submitter keyword: mass spectrometry,Acipenser baerii, ovarian fluid, eggs, proteome |
Contact List
Mariola A. Dietrich |
contact affiliation | Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland |
contact email | m.dietrich@pan.olsztyn.pl |
lab head | |
Agata Malinowska |
contact affiliation | IBB PAS |
contact email | esme@ibb.waw.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044168
- Label: PRIDE project
- Name: Comparative proteomic analysis of eggs and ovarian fluid in Siberian sturgeon: Insights into composition and functional significance