PXD044150

PXD044150 is an original dataset announced via ProteomeXchange.

Title	GSK3 phosphorylation sites in Lpd
Description	Neural crest cells are multipotent cells that delaminate from the neuroepithelium, migrating throughout the embryo. Aberrant migration causes developmental defects. Animal models are improving our understanding of neural crest anomalies, but in vivo migration behaviours are poorly understood. Here, we demonstrate that murine neural crest cells display actin-based lamellipodia and filopodia in vivo. Using neural crest-specific knockouts or inhibitors, we show that the serine-threonine kinase Glycogen Synthase Kinase-3 (GSK3), and the cytoskeletal regulator Lamellipodin (Lpd), are required for lamellipodia formation whilst preventing focal adhesion maturation. Lpd is a novel substrate of GSK3 and phosphorylation of Lpd favours interactions with the Scar/WAVE complex (lamellipodia formation) at the expense of VASP and Mena interactions (adhesion maturation and filopodia formation). This improved understanding of cytoskeletal regulation in mammalian neural crest migration has general implications for neural crest anomalies and cancer.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:52:29.971.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044150
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Steven Lynham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-27 10:53:20	ID requested
1	2023-10-05 04:10:01	announced
⏵ 2	2023-11-14 07:52:30	announced	2023-11-14: Updated project metadata.

10.6019/PXD044150;

submitter keyword: GSK3, phosphorylation, CID/ETD tandem mass spectrometry, Lpd

Karen J. Liu
contact affiliation	Centre for Craniofacial & Regenerative Biology, King’s College London/ Randall Centre for Cell & Molecular Biophysics, King’s College London
contact email	karen.liu@kcl.ac.uk
lab head
Steven Lynham
contact affiliation	King's College London
contact email	steven.lynham@kcl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044150
     1. Label: PRIDE project
     2. Name: GSK3 phosphorylation sites in Lpd