PXD044105 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Shotgun analysis of proteins in USP21 knock-out and WT HAP-1 cells |
Description | Deubiquitinases (DUBs) play a role in regulating protein degradation which is critical for maintaining protein homeostasis. The purpose of this study was to verify biological consequence of genetic depletion of USP21 in human cells on proteome changes and cellular function. Comparison of proteomic analysis of HAP-1 USP21 knockout and HAP-1 WT cells revealed that down-regulated proteins in HAP-1 USP21 KO cell are mostly engaged in mitochondrial function and ATP production. Cellular assays confirmed disturbance of mitochondrial metabolism and decrease in ATP production in HAP-1 USP21 knock-out cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_07:00:23.472.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Remigiusz Serwa |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-07-26 08:08:58 | ID requested | |
1 | 2024-10-02 04:23:22 | announced | |
⏵ 2 | 2024-10-22 07:00:23 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: cancer progression, ATP, mitochondrial respiration,USP21 |
Contact List
Dr Zbigniew Zaslona |
contact affiliation | Molecure SA, Warsaw, Poland |
contact email | z.zaslona@molecure.com |
lab head | |
Remigiusz Serwa |
contact affiliation | The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences |
contact email | r.serwa@imol.edu.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044105
- Label: PRIDE project
- Name: Shotgun analysis of proteins in USP21 knock-out and WT HAP-1 cells