PXD044105

PXD044105 is an original dataset announced via ProteomeXchange.

Title	Shotgun analysis of proteins in USP21 knock-out and WT HAP-1 cells
Description	Deubiquitinases (DUBs) play a role in regulating protein degradation which is critical for maintaining protein homeostasis. The purpose of this study was to verify biological consequence of genetic depletion of USP21 in human cells on proteome changes and cellular function. Comparison of proteomic analysis of HAP-1 USP21 knockout and HAP-1 WT cells revealed that down-regulated proteins in HAP-1 USP21 KO cell are mostly engaged in mitochondrial function and ATP production. Cellular assays confirmed disturbance of mitochondrial metabolism and decrease in ATP production in HAP-1 USP21 knock-out cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:00:23.472.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-26 08:08:58	ID requested
1	2024-10-02 04:23:22	announced
⏵ 2	2024-10-22 07:00:23	announced	2024-10-22: Updated project metadata.

10.1016/J.JBC.2024.107793;

submitter keyword: cancer progression, ATP, mitochondrial respiration,USP21

Dr Zbigniew Zaslona
contact affiliation	Molecure SA, Warsaw, Poland
contact email	z.zaslona@molecure.com
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD044105

PRIDE project URI

• PRIDE
  1. PXD044105
     1. Label: PRIDE project
     2. Name: Shotgun analysis of proteins in USP21 knock-out and WT HAP-1 cells