PXD044099

PXD044099 is an original dataset announced via ProteomeXchange.

Title	Liver proteome of Mus musculus
Description	Acetaminophen is a widely used antipyretic and analgesic drug, and its overdose is the leading cause of drug-induced acute liver failure. This study aimed to investigate the effect and mechanism of Lacticaseibacillus casei Shirota (LcS), an extensively used and highly studied probiotic, on acetaminophen-induced acute liver injury. C57BL/6 mice were gavaged with LcS suspension or saline once daily for 7 days before the acute liver injury was induced via intraperitoneal injection of 300 mg/kg acetaminophen. The results showed that LcS significantly decreased acetaminophen-induced liver and ileum injury, as demonstrated by reductions in the increases in aspartate aminotransferase, total bile acids, total bilirubin, indirect bilirubin and hepatic cell necrosis. Moreover, LcS alleviated the acetaminophen-induced intestinal mucosal permeability, elevation in serum IL-1α and lipopolysaccharide, and decreased levels of serum eosinophil chemokine (eotaxin) and hepatic glutathione levels. Furthermore, analysis of the gut microbiota and metabolome showed that LcS reduced the acetaminophen-enriched levels of Cyanobacteria, Oxyphotobacteria, long-chain fatty acids, cholesterol and sugars in the gut. Additionally, the transcriptome and proteomics showed that LcS mitigated the downregulation of metabolism and immune pathways as well as glutathione formation during acetaminophen-induced acute liver injury. This is the first study showing that pretreatment with LcS alleviates acetaminophen-enriched acute liver injury, and it provides a reference for the application of LcS.
HostingRepository	iProX
AnnounceDate	2023-07-26
AnnouncementXML	Submission_2024-02-27_18:41:41.231.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ying Yu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-26 03:34:14	ID requested
1	2023-07-26 03:35:44	announced
⏵ 2	2024-02-27 18:41:42	announced	2024-02-28: Update information.

Lv L, Ren S, Jiang H, Yan R, Chen W, Yan R, Dong J, Shao L, Yu Y, Shirota alleviates acetaminophen-induced liver injury through accelerated acetaminophen metabolism via the liver-gut axis in mice. mSphere, 9(1):e0067223(2024) [pubmed]

submitter keyword: liver, proteome, Mus musculus, Lacticaseibacillus casei Shirota, Acetaminophen

Ying Yu
contact affiliation	Hangzhou Medical College
contact email	yuying@hmc.edu.cn
lab head
Ying Yu
contact affiliation	Hangzhou Medical College
contact email	yuying@hmc.edu.cn
dataset submitter

iProX dataset URI