PXD044094
PXD044094 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | An axonemal intron splicing program sustains Plasmodium male development |
Description | To gain further insights into the post-transcriptional regulation process in the male sexual stage of Plasmodium, we focused on RBPm1, the most specifically expressed RNA-binding protein in the male gametocyte of P. yoelii, for in-depth investigation. In the first part of this study, we identified RBPm1 as a splicing activator, facilitating the splicing of 30 introns among 26 genes in the male gametocytes. Next, we sought to elucidate how RBPm1 exerts its splicing-promoting function. To achieve this, we performed biotin-based proximity labeling experiments on RBPm1, identifying 113 proteins that interact with RBPm1. Among these proteins, all 13 subunits of the spliceosome E complex were present, while other components of the splicing cycle were not significantly enriched. This suggests that RBPm1 participates in the initiation step of RNA splicing by interacting with the E-complex to promote intron splicing. |
HostingRepository | iProX |
AnnounceDate | 2023-07-26 |
AnnouncementXML | Submission_2025-05-21_18:50:10.925.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peijia Wu |
SpeciesList | scientific name: Plasmodium yoelii yoelii 17XNL; NCBI TaxID: 352914; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-07-25 21:03:32 | ID requested | |
1 | 2023-08-23 19:48:40 | announced | |
⏵ 2 | 2025-05-21 18:50:11 | announced | 2025-05-22: Add Publication. |
Publication List
Guan J, Wu P, Mo X, Zhang X, Liang W, Zhang X, Jiang L, Li J, Cui H, Yuan J, An axonemal intron splicing program sustains Plasmodium male development. Nat Commun, 15(1):4697(2024) [pubmed] |
Keyword List
submitter keyword: RNA binding protein, spliceosome, malaria |
Contact List
Jing Yuan | |
---|---|
contact affiliation | Xiamen University |
contact email | yuanjing@xmu.edu.cn |
lab head | |
Peijia Wu | |
contact affiliation | Xiamen University |
contact email | 21620220156999@stu.xmu.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |