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PXD044094

PXD044094 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn axonemal intron splicing program sustains Plasmodium male development
DescriptionTo gain further insights into the post-transcriptional regulation process in the male sexual stage of Plasmodium, we focused on RBPm1, the most specifically expressed RNA-binding protein in the male gametocyte of P. yoelii, for in-depth investigation. In the first part of this study, we identified RBPm1 as a splicing activator, facilitating the splicing of 30 introns among 26 genes in the male gametocytes. Next, we sought to elucidate how RBPm1 exerts its splicing-promoting function. To achieve this, we performed biotin-based proximity labeling experiments on RBPm1, identifying 113 proteins that interact with RBPm1. Among these proteins, all 13 subunits of the spliceosome E complex were present, while other components of the splicing cycle were not significantly enriched. This suggests that RBPm1 participates in the initiation step of RNA splicing by interacting with the E-complex to promote intron splicing.
HostingRepositoryiProX
AnnounceDate2023-07-26
AnnouncementXMLSubmission_2025-05-21_18:50:10.925.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPeijia Wu
SpeciesList scientific name: Plasmodium yoelii yoelii 17XNL; NCBI TaxID: 352914;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-25 21:03:32ID requested
12023-08-23 19:48:40announced
22025-05-21 18:50:11announced2025-05-22: Add Publication.
Publication List
Guan J, Wu P, Mo X, Zhang X, Liang W, Zhang X, Jiang L, Li J, Cui H, Yuan J, An axonemal intron splicing program sustains Plasmodium male development. Nat Commun, 15(1):4697(2024) [pubmed]
Keyword List
submitter keyword: RNA binding protein, spliceosome, malaria
Contact List
Jing Yuan
contact affiliationXiamen University
contact emailyuanjing@xmu.edu.cn
lab head
Peijia Wu
contact affiliationXiamen University
contact email21620220156999@stu.xmu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI