PXD044094

PXD044094 is an original dataset announced via ProteomeXchange.

Title	An axonemal intron splicing program sustains Plasmodium male development
Description	To gain further insights into the post-transcriptional regulation process in the male sexual stage of Plasmodium, we focused on RBPm1, the most specifically expressed RNA-binding protein in the male gametocyte of P. yoelii, for in-depth investigation. In the first part of this study, we identified RBPm1 as a splicing activator, facilitating the splicing of 30 introns among 26 genes in the male gametocytes. Next, we sought to elucidate how RBPm1 exerts its splicing-promoting function. To achieve this, we performed biotin-based proximity labeling experiments on RBPm1, identifying 113 proteins that interact with RBPm1. Among these proteins, all 13 subunits of the spliceosome E complex were present, while other components of the splicing cycle were not significantly enriched. This suggests that RBPm1 participates in the initiation step of RNA splicing by interacting with the E-complex to promote intron splicing.
HostingRepository	iProX
AnnounceDate	2023-07-26
AnnouncementXML	Submission_2025-05-21_18:50:10.925.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peijia Wu
SpeciesList	scientific name: Plasmodium yoelii yoelii 17XNL; NCBI TaxID: 352914;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-25 21:03:32	ID requested
1	2023-08-23 19:48:40	announced
⏵ 2	2025-05-21 18:50:11	announced	2025-05-22: Add Publication.

Guan J, Wu P, Mo X, Zhang X, Liang W, Zhang X, Jiang L, Li J, Cui H, Yuan J, An axonemal intron splicing program sustains Plasmodium male development. Nat Commun, 15(1):4697(2024) [pubmed]

submitter keyword: RNA binding protein, spliceosome, malaria

Jing Yuan
contact affiliation	Xiamen University
contact email	yuanjing@xmu.edu.cn
lab head
Peijia Wu
contact affiliation	Xiamen University
contact email	21620220156999@stu.xmu.edu.cn
dataset submitter

iProX dataset URI