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PXD044091

PXD044091 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleImmunoprecipitation of CLAMP-HA and CLAMPΔPRD-HA from the parasite T. gondii
DescriptionApicomplexan parasites discharge specialized organelles called rhoptries upon host cell contact to mediate invasion. The events that drive rhoptry discharge are poorly understood, yet essential to sustain the apicomplexan parasitic life cycle. Rhoptry discharge appears to depend on proteins secreted from another set of organelles called micronemes, which in Toxoplasma gondii includes MIC8 and the microneme-associated CRMP complex. Here, we examine the function of the microneme protein CLAMP, uncovering its essential role in rhoptry discharge. CLAMP forms a distinct complex with two other microneme proteins, the invasion-associated SPATR, and a previously uncharacterized protein we name CLAMP-linked invasion protein (CLIP). Deletion of CLAMP’s cytosolic region does not disrupt complex association, revealing this complex resides in micronemes or secreted to the surface of parasites. CLAMP-deficiency does not impact parasite adhesion or microneme protein secretion; however, knockdown of any member of the CLAMP complex affects rhoptry discharge. Phylogenetic analysis suggests orthologs of the essential complex components, CLAMP and CLIP, are ubiquitous across apicomplexans. Nevertheless, SPATR, which appears to act as an accessory factor in Toxoplasma, is essential for invasion during Plasmodium falciparum blood stages. Our results reveal a new protein complex that mediates rhoptry discharge following host-cell contact.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:13:42.405.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD044091
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDylan Valleau
SpeciesList scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601;
ModificationListphosphorylated residue; myristoylated residue; monohydroxylated residue; deamidated residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-25 20:18:38ID requested
12023-10-30 07:29:06announced
22023-11-14 09:13:45announced2023-11-14: Updated project metadata.
Publication List
10.6019/PXD044091;
Valleau D, Sidik SM, Godoy LC, Acevedo-S, á, nchez Y, Pasaje CFA, Huynh MH, Carruthers VB, Niles JC, Lourido S, A conserved complex of microneme proteins mediates rhoptry discharge in Toxoplasma. EMBO J, 42(23):e113155(2023) [pubmed]
Keyword List
submitter keyword: Rhoptry secretion.,Toxoplasma gondii, CLAMP
Contact List
Sebastian Lourido
contact affiliationWhitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Biology, Cambridge, MA, USA
contact emaillourido@wi.mit.edu
lab head
Dylan Valleau
contact affiliationWhitehead Institute for Biomedical Research
contact emailvalleau@wi.mit.edu
dataset submitter
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Dataset FTP location
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