PXD044090

PXD044090 is an original dataset announced via ProteomeXchange.

Title	Lactylome analysis reveals HADHA lactylation controls energy homeostasis to promote sepsis-induced myocardial depression.
Description	Serum lactate levels are interpreted as a marker of tissue hypoxia and often used clinically as an indicator of severity and outcome of patients with sepsis/septic shock. Glycolysis-derived lactate was identified as a substrate for lysine lactylation (Kla), which is a recently described type of posttranslational modification. However, the impact of lactylation on biological processes of sepsis-induced myocardial depression remains largely unknown. Here, we conduct lactylome and proteome profiling, and quantify 1,127 Kla sites, of which 83 are differentially lactated in sepsis-induced myocardial depression. We develop a deep learning-based framework to prioritize the lactylation at K166 and K728 of trifunctional enzyme subunit alpha (HADHA) to be functionally important. We perform validations to show that lactylation of HADHA at K166 and K728 is influenced both by endogenous and exogenous lactate, and further verify that lactylation at K166 and K728 inhibits the activity of HADHA, decreasing ATP production, an
HostingRepository	iProX
AnnounceDate	2023-07-26
AnnouncementXML	Submission_2023-07-25_19:45:23.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tiening Zhang
SpeciesList	scientific name: Rattus rattus; NCBI TaxID: 10117;
ModificationList	lactic acid
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-25 19:45:05	ID requested
⏵ 1	2023-07-25 19:45:24	announced

Dataset with its publication pending

submitter keyword: Lactylation, metabolism, sepsis, heart dysfunction, lactylome

Tiening Zhang
contact affiliation	Shengjing Hospital of China Medical University
contact email	cmuztn@vip.qq.com
lab head
Tiening Zhang
contact affiliation	Shengjing Hospital of China Medical University
contact email	cmuztn@vip.qq.com
dataset submitter

iProX dataset URI