PXD044056
PXD044056 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A Hidden Human Proteome Encoded by the “Non-Coding” Genes |
| Description | It has been a long debate whether the 98% “non-coding” fraction of human genome can encode functional proteins besides a “random noise” of translation. We used our established translatome sequencing (RNC-seq) to analyze human cells and found that up to 3330 long non-coding RNAs (lncRNAs) were bound to ribosomes and thus might be translated into proteins (with more than 50 amino acids). These new protein-coding genes distributed universally in all human chromosomes. We then used various experimental methods including mass spectrometry, immunoblotting, subcellular localization and phenotype assessments to verify the existence of such a hidden human proteome encoded by purported lncRNAs that can express functional proteins. These new proteins deviate from the canonical proteins in various physical and chemical properties, and emerged mostly in primates during evolution. In sum, we experimentally evidenced a hidden human functional proteome encoded by purported lncRNAs, suggesting that the human genome has to be systematically re-annotated. |
| HostingRepository | iProX |
| AnnounceDate | 2019-08-29 |
| AnnouncementXML | Submission_2023-07-24_21:10:53.917.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhang Jing |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | TripleTOF 5600; QTRAP 6500+; Orbitrap Fusion; TSQ Quantiva |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-07-24 21:10:41 | ID requested | |
| ⏵ 1 | 2023-07-24 21:10:54 | announced |
Publication List
| Lu S, Zhang J, Lian X, Sun L, Meng K, Chen Y, Sun Z, Yin X, Li Y, Zhao J, Wang T, Zhang G, He QY, A hidden human proteome encoded by 'non-coding' genes. Nucleic Acids Res, 47(15):8111-8125(2019) [pubmed] |
Keyword List
| submitter keyword: Proteome, lncRNAs, HPP, Translatome |
Contact List
| Qing-Yu He | |
|---|---|
| contact affiliation | Jinan University |
| contact email | tqyhe@email.jnu.edu.cn |
| lab head | |
| Zhang Jing | |
| contact affiliation | Jinan University |
| contact email | 1047596410@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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