PXD044045

PXD044045 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic characterization of extracellular vesicles (EVs) released by primary human vascular smooth muscle cells (VSMCs) under calcification stress
Description	Vascular calcification (VC) is a strong predictor of cardiovascular risk, particularly in chronic kidney disease (CKD) patients, associated with increased vascular stiffness, pulse pressure, left ventricular hypertrophy and atherosclerotic plaque burden [1]. VC is currently accepted as a highly controlled multifactorial process, where the release of extracellular vesicles (EVs) with calcification capacity plays an essential role in mediating cell-induced matrix mineralization [2]. Still, questions considering mechanisms of EVs deposition in the extracellular space, and its relationship with vesicle origin, loading, calcifying capacity and role in intercellular communication remain elusive. In this work we established a proteomic approach to characterize extracellular matrix (ECM)-deposited and cell media (CM) released EVs, from an in vitro model of VC consisting of primary vascular smooth muscle cells (VSMCs), bringing new knowledge into their specific characteristics and cargo.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_10:13:59.309.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044045
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Teresa Mendes Maia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-24 08:54:17	ID requested
⏵ 1	2025-05-06 10:14:00	announced

Publication List

10.6019/PXD044045;
10.3390/ijms252212406;
Viegas C, Carreira J, Maia TM, Macedo AL, Matos AP, Neves J, Simes D, Gla Rich Protein (GRP) Mediates Vascular Smooth Muscle Cell (VSMC) Osteogenic Differentiation, Extracellular Vesicle (EV) Calcification Propensity, and Immunomodulatory Properties. Int J Mol Sci, 25(22):(2024) [pubmed]

10.6019/PXD044045;

10.3390/ijms252212406;

Viegas C, Carreira J, Maia TM, Macedo AL, Matos AP, Neves J, Simes D, Gla Rich Protein (GRP) Mediates Vascular Smooth Muscle Cell (VSMC) Osteogenic Differentiation, Extracellular Vesicle (EV) Calcification Propensity, and Immunomodulatory Properties. Int J Mol Sci, 25(22):(2024) [pubmed]

Keyword List

submitter keyword: Human
Vascular smooth muscle cells (VSMCs)
vascular calcification
LC-MSMS

submitter keyword: Human

Vascular smooth muscle cells (VSMCs)

vascular calcification

LC-MSMS

Contact List

Carla Viegas; Dina Simes
contact affiliation	University of Algarve, Centre of Marine Sciences (CCMAR), Campus de Gambelas, 8005-139 Faro, Portugal
contact email	dsimes@ualg.pt
lab head
Teresa Mendes Maia
contact affiliation	VIB Proteomics Core
contact email	teresa.maia@vib-ugent.be
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD044045

PRIDE project URI

Repository Record List

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