PXD044045 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic characterization of extracellular vesicles (EVs) released by primary human vascular smooth muscle cells (VSMCs) under calcification stress |
Description | Vascular calcification (VC) is a strong predictor of cardiovascular risk, particularly in chronic kidney disease (CKD) patients, associated with increased vascular stiffness, pulse pressure, left ventricular hypertrophy and atherosclerotic plaque burden [1]. VC is currently accepted as a highly controlled multifactorial process, where the release of extracellular vesicles (EVs) with calcification capacity plays an essential role in mediating cell-induced matrix mineralization [2]. Still, questions considering mechanisms of EVs deposition in the extracellular space, and its relationship with vesicle origin, loading, calcifying capacity and role in intercellular communication remain elusive. In this work we established a proteomic approach to characterize extracellular matrix (ECM)-deposited and cell media (CM) released EVs, from an in vitro model of VC consisting of primary vascular smooth muscle cells (VSMCs), bringing new knowledge into their specific characteristics and cargo. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-06 |
AnnouncementXML | Submission_2025-05-06_10:13:59.309.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD044045 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Teresa Mendes Maia |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-07-24 08:54:17 | ID requested | |
⏵ 1 | 2025-05-06 10:14:00 | announced | |
Publication List
10.6019/PXD044045; |
10.3390/ijms252212406; |
Viegas C, Carreira J, Maia TM, Macedo AL, Matos AP, Neves J, Simes D, Gla Rich Protein (GRP) Mediates Vascular Smooth Muscle Cell (VSMC) Osteogenic Differentiation, Extracellular Vesicle (EV) Calcification Propensity, and Immunomodulatory Properties. Int J Mol Sci, 25(22):(2024) [pubmed] |
Keyword List
submitter keyword: Human |
Vascular smooth muscle cells (VSMCs) |
vascular calcification |
LC-MSMS |
Contact List
Carla Viegas; Dina Simes |
contact affiliation | University of Algarve, Centre of Marine Sciences (CCMAR), Campus de Gambelas, 8005-139 Faro, Portugal |
contact email | dsimes@ualg.pt |
lab head | |
Teresa Mendes Maia |
contact affiliation | VIB Proteomics Core |
contact email | teresa.maia@vib-ugent.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044045
- Label: PRIDE project
- Name: Proteomic characterization of extracellular vesicles (EVs) released by primary human vascular smooth muscle cells (VSMCs) under calcification stress