PXD044040

PXD044040 is an original dataset announced via ProteomeXchange.

Title	Protein modifications in plasma during SARS-CoV-2 infection
Description	Although the COVID-19 pandemic has ended, it is important to understand the pathology of severe SARS-CoV-2 infection associated with respiratory failure and high mortality. In this study, a proteomics approach using nano-HPLC-MS/MS (QExactive HF) was used to compare the plasma proteome of COVID-19 survivors (COVID-19; n=10) and deceased individuals (CovDeath; n=10) with that of healthy individuals (Ctr; n=10). The effects of SARS-CoV-2 infection on the alteration of plasma proteins by lipid peroxidation products such as 4-hydroxynonenal (4-HNE), malondialdehyde (MDA) and 15-deoxy-12,14-prostaglandin J2 (15d-PGJ2) were also examined. The results suggest that the development of COVID-19 strongly alters the expression of proteins involved in the regulation of exocytosis and platelet degranulation. These changes were most pronounced in the CovDeath group. In addition, a significant increase in proteins modified with reactive aldehydes was observed in all patients. In the COVID-19 and CovDeath groups, the levels of 4-HNE adducts increased 2- and 3-fold, respectively, whereas MDA adducts increased 7- and 2.5-fold, respectively. Signaling kinases and proinflammatory proteins were particularly affected by these modifications, and the amount of 4-HNE modifications confirmed previous findings on the relevance of 4-HNE in disease pathogenesis and lethal outcome. Protein adducts with 15d-PGJ2 were increased 2.5-fold in COVID-19 patients, including modifications of proteins such as p53 and STAT3, whereas CovDeath showed a decrease of approximately 60% compared with Ctr. It can be assumed that the observed changes in protein expression and modification in COVID-19 are unlikely to be used as prognostic biomarkers because they are also present in the other inflammatory diseases. However, larger studies may prove that the extent and the nature of protein modifications in plasma may be a predictor of the course of SARS-CoV-2 infection.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:21.575.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Agnieszka Gęgotek
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-24 08:16:13	ID requested
1	2023-10-24 07:49:00	announced
⏵ 2	2023-11-14 09:06:23	announced	2023-11-14: Updated project metadata.

G, ę, gotek A, Zarkovic N, Orehovec B, Jaganjac M, Sunjic SB, Skrzydlewska E, Short Survey on the Protein Modifications in Plasma during SARS-CoV-2 Infection. Int J Mol Sci, 24(18):(2023) [pubmed]

submitter keyword: 14-prostaglandin J2,COVID-19

plasma proteome

protein adducts

4-hydroxynonenal

malondialdehyde

15-deoxy-12

Agnieszka Gegotek
contact affiliation	UMB
contact email	agnieszka.gegotek@umb.edu.pl
lab head
Agnieszka Gęgotek
contact affiliation	Medical University of Białystok
contact email	agnieszkagegotek@o2.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD044040

PRIDE project URI

• PRIDE
  1. PXD044040
     1. Label: PRIDE project
     2. Name: Protein modifications in plasma during SARS-CoV-2 infection