PXD044039

PXD044039 is an original dataset announced via ProteomeXchange.

Title	Low-invasive sampling method for taxonomic for the identification of archaeological and paleontological bone by proteomics of their collagens
Description	Bone collagen is an important organic material for isotopic measurement, radiocarbon and paleoproteomic analyzes, to provide information on diet, dating, taxonomic identification. Current paleoproteomics methods are destructive and require from a few milligrams to several tenths of milligrams of bone for analysis. In many cultures, bones are raw materials for artefact which are conserved in museum which hampers to damage these precious objects during sampling. Here, we describe a minimal sampling method that identifies collagen, taxonomy and post-translational modifications from Holocene and Upper Pleistocene bones dated to 130,000 and 5,000 years ago using dermatological skin tape-discs for sampling. The sampled bone micro-powder were digested following our highly optimized eFASP protocol, then analyzed by MALDI FTICR MS and LC-MS/MS for identifying the genus taxa of the bones. We show that this low-invasive sampling does not deteriorate the bones and achieves results similar to those obtained by destructive sampling. Moreover, this sampling method can be performed at archaeological sites or in museums.
HostingRepository	PRIDE
AnnounceDate	2024-07-25
AnnouncementXML	Submission_2024-07-25_09:52:56.800.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	BRAY FABRICE
SpeciesList	scientific name: Cervus elaphus (Red deer); NCBI TaxID: 9860; scientific name: Capra ibex (Ibex); NCBI TaxID: 72542; scientific name: Ursus sp.; NCBI TaxID: 9641; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Equus sp.; NCBI TaxID: 46122; scientific name: Castor fiber (Eurasian beaver); NCBI TaxID: 10185; scientific name: Struthio camelus (Ostrich); NCBI TaxID: 8801;
ModificationList	carbamoylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-24 08:13:17	ID requested
⏵ 1	2024-07-25 09:52:57	announced

10.1021/ACS.JPROTEOME.4C00083;

submitter keyword: MALDI FTICR MS, taxonomy,Palaeoproteomics, skin sampling tape discs, LC-MS/MS

fabrice bray
contact affiliation	​​Laboratoire MSAP UAR 3290 CNRS Bâtiment C4 Avenue Paul Langevin Cité Scientifique 59655 Villeneuve D'ascq 03 20 33 71 12 https://msap-lab.fr/
contact email	fabrice.bray@univ-lille.fr
lab head
BRAY FABRICE
contact affiliation	MSAP USR 3290
contact email	fabrice.bray@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD044039

PRIDE project URI

• PRIDE
  1. PXD044039
     1. Label: PRIDE project
     2. Name: Low-invasive sampling method for taxonomic for the identification of archaeological and paleontological bone by proteomics of their collagens