PXD044034

PXD044034 is an original dataset announced via ProteomeXchange.

Title	Post-translational modification of the transcription factor SPATULA by SECRET AGENT and SPINDLY promotes Arabidopsis style development.
Description	O-linked-β-N-Acetylglucosamine (O-GlcNAc) and O-fucose are two sugar-based post-translational modifications (PTMs) whose mechanistic role in plant signalling and transcriptional regulation is still largely unknown. Here, we investigated how two O-glycosyltransferase enzymes of Arabidopsis thaliana, SPINDLY (SPY) and SECRET AGENT (SEC) promote the activity of the bHLH transcription factor SPATULA (SPT), during morphogenesis of the plant female reproductive organ apex, the style. SPY and SEC modify N-terminal residues of SPT in vivo and in vitro by attaching O-fucose and O-GlcNAc, respectively. This post-translational regulation does not impact SPT homo- and hetero-dimerisation events, although it enhances the affinity of SPT for the kinase PINOID (PID) gene locus and its transcriptional repression. Our findings offer the first mechanistic example of the effect of O-GlcNAc and O-fucose on the activity of a plant transcription factor and reveal a previously unrecognized roles for SEC and SPY in orchestrating style elongation and shape.
HostingRepository	PRIDE
AnnounceDate	2024-01-22
AnnouncementXML	Submission_2024-01-22_12:03:01.253.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044034
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	N-acetylhexosaminylated residue; deoxyhexosylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-24 07:34:54	ID requested
⏵ 1	2024-01-22 12:03:01	announced

10.6019/PXD044034;

submitter keyword: in vitro, proteomics, SPY, mass spectrometry, SPT,O-glycosylation, gynoecium, SEC

Laila Moubayidin
contact affiliation	Group Leader Cell&Developmental Biology John Innes Centre Norwich, UK
contact email	Laila.Moubayidin@jic.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044034
     1. Label: PRIDE project
     2. Name: Post-translational modification of the transcription factor SPATULA by SECRET AGENT and SPINDLY promotes Arabidopsis style development.