PXD044033
PXD044033 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics of tumor associated microglia, tumor tissue from SB28 glioblastoma mice, and control microglia |
Description | The 18 kDa translocator protein (TSPO) emerges as an important PET biomarker to assess the tumor microenvironment (TME) in glioblastoma. However, various cellular sources hamper interpretation and biological understanding of TSPO and other immune biomarkers in the TME. Thus, we established a novel method, combining immunomagnetic cell sorting after radiotracer injection (scRadiotracing) with 3D histology via light sheet microscopy and proteomics to dissect cellular allocation of TSPO enrichment in glioblastoma. Single tumor cells of implanted SB28 glioblastoma mice indicated 1.37-fold higher TSPO tracer uptake and 1.46-fold higher TSPO protein expression levels when compared to tumor associated microglia/macrophages (TAMs). Using proteomics, we compared the proteome of tumor associated microglia/macrophages (TAMs), Tumor tissue (TT) and control microglia from WT mice without glioblastoma. This analysis identified TAM specific targets for PET radioligand development with additional potential to monitor diverse TAM subpopulations in vivo. In summary, our data indicate that tumor cells need to be acknowledged as the main contributor to TSPO as a biomarker in glioblastoma. Combining cellular tracer uptake measures with 3D histology facilitates precise allocation of complex PET signal sources and will serve to validate novel TAM specific radioligands. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:13:35.790.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stephan Mueller |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-07-24 07:09:24 | ID requested | |
1 | 2023-10-30 02:53:01 | announced | |
⏵ 2 | 2023-11-14 09:13:41 | announced | 2023-11-14: Updated project metadata. |
Publication List
Bartos LM, Kirchleitner SV, Kolabas ZI, Quach S, Beck A, Lorenz J, Blobner J, Mueller SA, Ulukaya S, Hoeher L, Horvath I, Wind-Mark K, Holzgreve A, Ruf VC, Gold L, Kunze LH, Kunte ST, Beumers P, Park HE, Antons M, Zatcepin A, Briel N, Hoermann L, Schaefer R, Messerer D, Bartenstein P, Riemenschneider MJ, Lindner S, Ziegler S, Herms J, Lichtenthaler SF, Ert, ΓΌ, rk A, Tonn JC, von Baumgarten L, Albert NL, Brendel M, Deciphering sources of PET signals in the tumor microenvironment of glioblastoma at cellular resolution. Sci Adv, 9(43):eadi8986(2023) [pubmed] |
Keyword List
submitter keyword: tumor tissue, microglia,tumor associated microglia, SB28 glioblastoma |
Contact List
Stefan F. Lichtenthaler | |
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contact affiliation | DZNE Munich, Neuroproteomics, Feodor-Lynen Str. 17, 81377 Munich, Germany |
contact email | stefan.lichtenthaler@dzne.de |
lab head | |
Stephan Mueller | |
contact affiliation | DZNE Munich Neuroproteomics |
contact email | stephan.mueller@dzne.de |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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