PXD044027

PXD044027 is an original dataset announced via ProteomeXchange.

Title	Hierarchical learning reveals ketogenic diet reprograms cancer metabolism through lysine β-hydroxybutyrylation
Description	To investigate the impact of ketogenesis, we subjected 3-month-old mice with either a ketogenic diet (KD) or a control diet (Ctrl) group for 9 weeks. Similar to previous studies, KD led to a significantly decreased mice weight (Fig. 1b). Furthermore, adherence to a KD dramatically elevated blood β-OHB <<28877458/28877456>> and decreased blood glucose <33838492> (Fig. 1c, d). To investigate how KD affected mice liver proteomes post-translationally, we probed the liver tissues with various pan-PTM antibodies. KD dramatically increased the global levels of Kbhb, acetylation (Kac), and O-GlcNAcylation, with the level of Kbhb increased most (6 fold relative to control). On the other hand, KD led to the decreased levels of lactylation and succinylation, but did not alter the levels of phosphorylation, ubiquitination and sumoylation.For label-free proteomic and β-hydroxybutyrylomic quantifications, 6 liver samples of Ctrl or KD mice were pooled into two samples, and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). For metabolomic profilling, 6 liver samples from Ctrl or KD mice were also adopted for untargeted metabolomic profiling in Ctrl and KD mice, respectively.
HostingRepository	iProX
AnnounceDate	2023-07-24
AnnouncementXML	Submission_2023-07-24_04:19:55.565.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinhe Huang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro; 6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-24 04:19:47	ID requested
⏵ 1	2023-07-24 04:19:55	announced

Dataset with its publication pending

submitter keyword: lysine β-hydroxybutyrylation, Kbhb, metabolomic, proteomics

Yu Xue
contact affiliation	Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology and the Collaborative Innovation Center for Brain Science, Huazhong University of Science and Technology
contact email	xueyu@hust.edu.cn
lab head
Xinhe Huang
contact affiliation	Huazhong University of Science and Technology
contact email	hxhdddh2@gmail.com
dataset submitter

iProX dataset URI