PXD044014

PXD044014 is an original dataset announced via ProteomeXchange.

Title	Mycobacterium tuberculosis, LC-MS/MS
Description	Antibiotic resistance in Mycobacterium tuberculosis (Mtb) is a major public health problem worldwide. Therefore, it is of great significance to study the mutational pathways of susceptive Mtb to drug resistance. Multi-omics approach is ideal to explore more about other molecular mechanisms implicated in drug resistance. In this study, we respectively constructed continuous capreomycin (CAP) and amikacin (AKM)-resistant strain models in vitro using the increasing drug concentration gradients. Based on these models, we use multi-omics techniques and biological experiments to reveal the drug resistance mutations caused by CAP or AKM, and obtain other factors that can cause drug resistance besides the mutations, and explain the differences in cross-resistance pathways caused by AKM and CAP. Our results showed that in addition to Gly232AsptlyA and Trp120fstlyA directly associated with low level resistance to CAP, some mutations, including Ala48ValmmaA2, Gln19ArgrpmA and eis (c.-14c>t), were associated with cross-resistance caused by CAP. The transcriptome-proteome-lipid metabolome analysis of representative induced strains showed that cross-resistance of CAP-induced strains was caused by a combination of many aspects, which was obviously different from the induction of AKM. The results of this study will advance our understanding of the CAP-resistant mechanisms. To a certain extent, it is also conducive to improve the clinical understanding of capreomycin and rational use of CAP.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-14_16:12:48.772.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuchuan Zhao
SpeciesList	scientific name: Mycobacterium tuberculosis complex sp. AY1MRC; NCBI TaxID: NEWT:2664891;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-23 23:22:21	ID requested
⏵ 1	2025-12-14 16:12:49	announced

10.1016/j.crmicr.2025.100507;

Zhao Y, Zhang C, Wang X, Feng H, Yu M, Chen X, Liang J, Hong J, Huang S, Lin Y, Chen Y, Wei W, . Curr Res Microb Sci, 9():100507(2025) [pubmed]

submitter keyword: Mycobacterium tuberculosis

Yu chuan.Zhao
contact affiliation	Center for Tuberculosis Control of Guangdong Province, Guangzhou, 510630, China
contact email	zyc0934130326@163.com
lab head
Yuchuan Zhao
contact affiliation	Center for Tuberculosis Control of Guangdong Province, Guangzhou, 510630, China
contact email	zyc0934130326@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD044014

PRIDE project URI

• PRIDE
  1. PXD044014
     1. Label: PRIDE project
     2. Name: Mycobacterium tuberculosis, LC-MS/MS