PXD044013

PXD044013 is an original dataset announced via ProteomeXchange.

Title	Proteome and Ubiquitylome Analyses of Maize Endoplasmic Reticulum Under Heat Stress
Description	High temperatures severely affect plant growth and pose a threat to global crop production. Heat causes the accumulation of misfolded proteins in the ER, and triggers the heat shock response (HSR) in the cytosol and the unfolded protein response (UPR) in the ER. Excessive misfolded proteins undergo further degradation through ER associated degradation (ERAD). Although much work has been done on the plant heat stress response, the regulation of ER-localized proteins has not been well studied thus far. We isolated the microsome fraction from heat treated and untreated maize seedlings and performed proteomic and ubtiquitylome analyses. Out of 8306 total proteins detected by proteomics, 1675 proteins were significantly unregulated, and 708 proteins were significantly downregulated. Global ubiquitination analysis discovers 1780 proteins with at least one ubiquitination site in each protein. Motif analysis reveals that alanine and glycine are preferred upstream and downstream of the ubiquitinated lysine. ERAD components are found to be hyper-ubiquitinated after heat treatment, implying the feedback regulation of ERAD activity through protein degradation.
HostingRepository	iProX
AnnounceDate	2023-07-24
AnnouncementXML	Submission_2023-07-23_21:05:31.154.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chunyan Gao
SpeciesList	scientific name: Zea mays; NCBI TaxID: 4577;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Andromeda

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-23 21:04:42	ID requested
⏵ 1	2023-07-23 21:05:31	announced

Gao C, Peng X, Zhang L, Zhao Q, Ma L, Yu Q, Lian X, Gao L, Xiong L, Li S, Proteome and Ubiquitylome Analyses of Maize Endoplasmic Reticulum under Heat Stress. Genes (Basel), 14(3):(2023) [pubmed]

submitter keyword: heat stress,proteomics, ubiquitination, maize

Shengben Li
contact affiliation	Nanjing Agricultural University
contact email	shengbenli@njau.edu.cn
lab head
Chunyan Gao
contact affiliation	Nanjing Agricultural University
contact email	879514221@qq.com
dataset submitter

iProX dataset URI