PXD043988

PXD043988 is an original dataset announced via ProteomeXchange.

Title	HTLV-1 Rex hijacks UPF1 in a CRM1 dependent manner, leading to NMD inhibition and revealing proviral roles of UPF1.
Description	The hijacking of CRM1 export is an important step of the retroviral replication cycle. Here, we investigated the consequences of this hijacking for the host. During HTLV-1 infection, we identified the formation of a complex composed of REX, CRM1 and the RNA helicase UPF1, leading to the nuclear retention of UPF1. We further showed how this mislocalization leads to the inhibition of the nonsense mediated mRNA decay (NMD), known to have an antiviral function. Corroborating these results, we described a similar process with Rev, the functional homolog of Rex from HIV-1. Unexpectedly, we also found that, for HTLV-1, this process is coupled with the specific loading of UPF1 onto vRNA, independently of NMD. In this latter context, UPF1 positively regulates several steps of the viral replication cycle, from the nuclear export of vRNA to the production of mature viral particles.
HostingRepository	PRIDE
AnnounceDate	2025-05-14
AnnouncementXML	Submission_2025-05-14_04:42:30.440.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MARIETTE MATONDO
SpeciesList	scientific name: HTLV-1 subtype A; NCBI TaxID: 402042;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-21 09:02:13	ID requested
⏵ 1	2025-05-14 04:42:30	announced

10.1093/NAR/GKAS434;

submitter keyword: mass spectrometry,HTLV-1, UPF1

Vincent Mocquet
contact affiliation	Laboratoire de biologie et modélisation de la cellule Ecole Normale Supérieure - Lyon, Université Claude Bernard Lyon 1, U1293, UMR5239, France
contact email	vincent.mocquet@ens-lyon.fr
lab head
MARIETTE MATONDO
contact affiliation	Pasteur
contact email	mariette.matondo@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD043988

PRIDE project URI

• PRIDE
  1. PXD043988
     1. Label: PRIDE project
     2. Name: HTLV-1 Rex hijacks UPF1 in a CRM1 dependent manner, leading to NMD inhibition and revealing proviral roles of UPF1.