PXD043956

PXD043956 is an original dataset announced via ProteomeXchange.

Title	Multimode Chromatography-based Techniques for High Purity Isolation of Extracellular Vesicles from Human Blood Plasma
Description	Extracellular vesicles (EVs) play a pivotal role in various biological pathways, such as immune responses and the progression of diseases, including cancer. However, it is challenging to isolate EVs at high purity from blood plasma and other biofluids due to their low abundance compared to more predominant biomolecular species such as lipoprotein particles and free protein complexes. Ultracentrifugation-based EV isolation, the current gold standard technique, cannot overcome this challenge due to the similar densities of such species. We developed several novel approaches to enrich EVs from plasma while depleting contaminating molecular species using multimode chromatography-based strategies. On average, we identified 716±68 proteins and 1054±35 proteins in EV isolates from 100 µL of plasma using multimode chromatography- and ultracentrifugation-based techniques, respectively. The developed methods resulted in similar EV isolates purity, providing significant advantages in simplicity, throughput, scalability, and applicability for various downstream analytical and potential clinical applications
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_17:54:16.222.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alan Zimmerman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-20 08:20:47	ID requested
⏵ 1	2025-08-03 17:54:16	announced

10.1002/jex2.70070;

, Articles. J Extracell Biol, 4(7):e70070(2025) [pubmed]

submitter keyword: size exclusion chromatography, mass spectrometry, multimode chromatography, plasma,Extracellular vesicles

Alexander Ivanov
contact affiliation	Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University
contact email	a.ivanov@northeastern.edu
lab head
Alan Zimmerman
contact affiliation	Northeastern University
contact email	zimmerman.alan@northeastern.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD043956

PRIDE project URI

• PRIDE
  1. PXD043956
     1. Label: PRIDE project
     2. Name: Multimode Chromatography-based Techniques for High Purity Isolation of Extracellular Vesicles from Human Blood Plasma