PXD043954

PXD043954 is an original dataset announced via ProteomeXchange.

Title	Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis Improved the Anti-MRSA Activity of Brevibacillus sp. SPR20
Description	Brevibacillus sp. SPR20 produced potentially antibacterial substances against MRSA. The synthe-sis of these substances is controlled by their biosynthetic gene clusters. Several mutagenesis methods are used to overcome the restriction of gene regulations when genetic information is ab-sent. Atmospheric and room temperature plasma (ARTP) is a powerful technique to make random mutagenesis for microbial strain improvement. This study utilized an argon-based ARTP to conduct the mutations on SPR20. The positive mutants of 40% occurred. The M27 mutant exhib-ited an increase in anti-MRSA activity when compared to the wild-type, by which the MIC values were 250500 and 500 g/mL, respectively. M27 had genetic stability because it exhibited con-stant activity throughout 15 generations. This mutant had similar morphology and antibiotic susceptibility to the wild-type. Comparative proteomic analysis identified some specific proteins that were upregulated in M27. These proteins were involved in the metabolism of amino acids, cell structure and movement, and catalytic enzymes. These might result in the enhancement of the anti-MRSA activity of the ARTP-treated SPR20 mutant. This study supports the ARTP technology to increase the production of valuable antibacterial agents.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:02:14.860.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sucheewin Krobthong
SpeciesList	scientific name: Brevibacillus sp.; NCBI TaxID: 1882945;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-20 07:28:46	ID requested
1	2023-08-31 06:30:43	announced
2	2023-11-14 09:04:49	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:02:15	announced	2024-10-22: Updated project metadata.

10.3390/IJMS241512016;

submitter keyword: atmospheric and room temperature plasma (ARTP)

anti-MRSA activity

Brevibacillus sp.

muta-genesis

proteomic analysis

strain improvement.

Apichart Atipairin
contact affiliation	School of Pharmacy, Walailak University, Nakhon Si Thammarat, 80161, Thailand
contact email	apichart.at@mail.wu.ac.th
lab head
Sucheewin Krobthong
contact affiliation	Mahidol University
contact email	sucheewin82@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD043954

PRIDE project URI

• PRIDE
  1. PXD043954
     1. Label: PRIDE project
     2. Name: Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis Improved the Anti-MRSA Activity of Brevibacillus sp. SPR20