PXD043944

PXD043944 is an original dataset announced via ProteomeXchange.

Title	The interactome of the UapA transporter reveals putative new players in anterograde membrane cargo trafficking: ΔartA: UapA-TurboID
Description	Neosynthesized plasma membrane (PM) proteins co-translationally translocate to the ER, concentrate at regions called ER-exit sites (ERes) and pack into COPII secretory vesicles which are sorted to the early-Golgi through membrane fusion. Following Golgi maturation, membrane cargoes reach the late-Golgi, from where they exit in clathrin-coated vesicles destined to the PM, directly or through endosomes. Post-Golgi membrane cargo trafficking also involves the cytoskeleton and the exocyst. The Golgi-dependent secretory pathway is thought to be responsible for the trafficking of all major membrane proteins. However, our recent findings in Aspergillus nidulans showed that several plasma membrane cargoes, such as transporters and receptors, follow a sorting route that seems to bypass Golgi functioning. To gain insight on membrane trafficking and specifically Golgi-bypass, here we used proximity dependent biotinylation (PDB) coupled with data-independent acquisition mass spectrometry (DIA-MS) for identifying transient interactors of the UapA transporter. Our assays, which included proteomes of wild-type and mutant strains affecting ER-exit or endocytosis (ΔartA: UapA-TurboID), identified both expected and novel interactions that might be physiologically relevant to UapA trafficking.
HostingRepository	PRIDE
AnnounceDate	2023-11-21
AnnouncementXML	Submission_2023-11-21_08:00:17.101.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans); NCBI TaxID: 227321;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-20 01:57:40	ID requested
⏵ 1	2023-11-21 08:00:19	announced

Georgiou X, Dimou S, Diallinas G, Samiotaki M, The interactome of the UapA transporter reveals putative new players in anterograde membrane cargo trafficking. Fungal Genet Biol, 169():103840(2023) [pubmed]

10.1016/j.fgb.2023.103840;

submitter keyword: ER-exit

COPII

COPI

traffic

fungi

A. nidulans, Golgi-bypass

UapA

Proximity Dependent Biotinylation

proteomics

George Diallinas
contact affiliation	1Department of Biology, National and Kapodistrian University of Athens, Panepistimioupolis, Athens, 15784, Greece. 2Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion, 70013, Greece.
contact email	diallina@biol.uoa.gr
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD043944

PRIDE project URI

• PRIDE
  1. PXD043944
     1. Label: PRIDE project
     2. Name: The interactome of the UapA transporter reveals putative new players in anterograde membrane cargo trafficking: ΔartA: UapA-TurboID