PXD043942

PXD043942 is an original dataset announced via ProteomeXchange.

Title	Temporary specific alternations in the nascent proteome of HEK293T cells in response to mitochondrial stress induced by carbonyl cyanide m-chlorophenyl hydrazine
Description	This dataset elucidates the remodeling of the nascent proteome before and upon global translation attenuation in response to transient mitochondrial depolarization by carbonyl cyanide m-chlorophenyl hydrazine (CCCP). During and following CCCP treatment, nascent proteome in short (30min) time windows was metabolically labelled with L-azidehomoalanine (AHA) using the BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) methodology. AHA incorporation sites were further derivatized with biotin-PEG4-alkyne by click chemistry upon cell lysis, proteins were digested with trypsin, nascent biotinylated peptides enriched and quantified.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:00:23.458.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-20 01:20:29	ID requested
1	2024-10-02 04:20:17	announced
⏵ 2	2024-10-22 07:00:23	announced	2024-10-22: Updated project metadata.

10.1016/J.CELREP.2024.114803;

submitter keyword: BONCAT, protein translation,mitochondrial stress, nascent proteome

Prof. Agnieszka Chacinska
contact affiliation	IMol Polish Academy of Sciences, Warsaw, Poland
contact email	a.chacinska@imol.institute
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD043942

PRIDE project URI

• PRIDE
  1. PXD043942
     1. Label: PRIDE project
     2. Name: Temporary specific alternations in the nascent proteome of HEK293T cells in response to mitochondrial stress induced by carbonyl cyanide m-chlorophenyl hydrazine