PXD043940

PXD043940 is an original dataset announced via ProteomeXchange.

Title	UMAD1 contributes to ESCRT-III dynamic subunit turnover during cytokinetic abscission
Description	Abscission is the final stage of cytokinesis whereby the midbody, a thin intercellular bridge, is resolved to separate the daughter cells. Cytokinetic abscission is mediated by the Endosomal Sorting Complex Required for Transport (ESCRT), a conserved membrane remodelling machinery. The midbody organiser CEP55 recruits early acting ESCRT factors such as ESCRT-I and ALIX, which subsequently initiate the formation of ESCRT-III polymers that sever the midbody. We now identify UMAD1 as an ESCRT-I subunit that facilitates abscission. UMAD1 selectively associates with VPS37C and VPS37B, supporting the formation of cytokinesis-specific ESCRT-I assemblies. TSG101 recruits UMAD1 to the site of midbody abscission, to stabilise the CEP55/ESCRT-I interaction. We further demonstrate that the UMAD1/ESCRT-I interaction facilitates the final step of cytokinesis. Paradoxically, UMAD1 and ALIX co-depletion has synergistic effects on abscission whilst ESCRT-III recruitment to the midbody is not inhibited. Importantly, we find that both UMAD1 and ALIX are required for the dynamic exchange of ESCRT-III subunits at the midbody. Therefore, UMAD1 reveals a key functional connection between ESCRT-I and ESCRT-III that is required for cytokinesis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:01:26.484.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043940
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	xiaoping yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-20 00:37:50	ID requested
1	2023-08-04 08:51:00	announced
⏵ 2	2023-11-14 07:01:27	announced	2023-11-14: Updated project metadata.

10.6019/PXD043940;

submitter keyword: Cytokinesis

midbody

ESCRT

membrane remodelling

Juan Martin-Serrano
contact affiliation	King’s College London
contact email	Juan.martin_serrano@kcl.ac.uk
lab head
xiaoping yang
contact affiliation	KCL
contact email	xiaoping.yang@kcl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043940
     1. Label: PRIDE project
     2. Name: UMAD1 contributes to ESCRT-III dynamic subunit turnover during cytokinetic abscission