PXD043914

PXD043914 is an original dataset announced via ProteomeXchange.

Title	Impact of light and thioredoxins on the landscape of the plant thiol-disulfide proteome
Description	Thiol-based redox regulation is a crucial post-translational mechanism to acclimate plants to changing light availability. Here, we conduct a biotin-switch-based redox proteomics study to systematically investigate dynamics of the thiol-redox network in response to temporal changes in light availability and across genotypes lacking parts of the NTRC/thioredoxin (Trx) systems in the chloroplast. Temporal dynamics revealed light leading to marked decreases in the oxidation states of 75 chloroplast proteins mainly involved in photosynthesis during the first 10 min, followed by their partial re-oxidation after 2-6 hours into the photoperiod. This involved f, m and x-type Trx proteins showing similar light-induced reduction-oxidation dynamics, while NTRC, 2-Cys-Prx and Trx y2 showed an opposing pattern, being more oxidized in the light, compared to the dark. In Arabidopsis trxf1f2, trxm1m2 or ntrc mutants, most protein candidates showed increased oxidation states, compared to the wild type, suggesting their light-dependent dynamics to be related to the NTRC/Trx network. In this context, deficiencies in f- and m-type Trxs were found to have different impacts on the thiol-redox proteome depending on the light environment, being higher in constant and fluctuating light, respectively, while NTRC deficiency having a strong influence in all light conditions. Results indicate the plant redox proteome to be subject to dynamic changes in reductive and oxidative pathways to cooperatively fine-tune photosynthetic and metabolic processes in the light. This involves f-type Trxs and NTRC to play a role in constant light conditions, while both m-type Trxs and NTRC being important to balance changes in protein redox-pattern during dynamic alterations in fluctuating light intensities.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:27:59.665.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frederik Sommer
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-19 06:33:06	ID requested
1	2024-01-29 01:52:47	announced
⏵ 2	2024-10-22 06:28:00	announced	2024-10-22: Updated project metadata.

10.1093/PLPHYS/KIAD669;

submitter keyword: Arabidopsis, Thioredoxin, RedOx-Proteomics

Peter Geigenberger
contact affiliation	Biozentrum der LMU München Pflanzenmetabolismus Großhaderner Str. 2-4 82152 Planegg-Martinsried Germany
contact email	geigenberger@bio.lmu.de
lab head
Frederik Sommer
contact affiliation	Technische Universität Kaiserslautern
contact email	frsommer@bio.uni-kl.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043914
     1. Label: PRIDE project
     2. Name: Impact of light and thioredoxins on the landscape of the plant thiol-disulfide proteome