PXD043909

PXD043909 is an original dataset announced via ProteomeXchange.

Title	Analysis of the GFP-labelled β-dystroglycan interactome in Hek-293 transfected cells reveals novel intracellular networks
Description	So far very little is known about the fine regulation of some of these intracellular interactions of β-DG and how they are perturbed in diseases. To start filling this gap, HEK-293 cells were transiently transfected with a plasmid carrying the β-DG subunit with GFP fused at its C-terminus. Immunoprecipitation by anti-GFP antibodies followed by shotgun proteomic analysis to investigate the proteins exclusively matching for β-DG binding, applying the following filters to MS identification data: high confidence, peptide rank 1, peptide length minimum 9 amino acid residues and selecting 2 peptides per protein. A series of already known β-DG interactors have been found, whilst significant new matches, which include potential novel β-DG interactors and their related networks, were identified in diverse subcellular compartments.
HostingRepository	PRIDE
AnnounceDate	2024-02-12
AnnouncementXML	Submission_2024-02-12_01:29:05.057.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Claudia Desiderio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-19 05:28:44	ID requested
⏵ 1	2024-02-12 01:29:05	announced

10.1016/J.BBRC.2024.149656;

submitter keyword: nuclear envelopathies, muscular dystrophy, Lamina-Associated Polypeptide-1B (LAP1B), mass spectrometry proteomics,dystroglycan, Torsin 1A Interacting Protein 1.

Claudia Desiderio, Andrea Brancaccio, Francesca Sciandra
contact affiliation	Consiglio Nazionale delle Ricerche, Istituto di Scienze e Tecnologie Chimiche (SCITEC) "Giulio Natta"
contact email	claudia.desiderio@cnr.it
lab head
Claudia Desiderio
contact affiliation	Consiglio Nazionale delle Ricerche - Istituto di Scienze e Tecnologie Chimiche (SCITEC) "Giulio Natta"
contact email	claudia.desiderio@cnr.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD043909

PRIDE project URI

• PRIDE
  1. PXD043909
     1. Label: PRIDE project
     2. Name: Analysis of the GFP-labelled β-dystroglycan interactome in Hek-293 transfected cells reveals novel intracellular networks