PXD043906

PXD043906 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of centrosomes across multiple cell and tissue types by a new affinity capture method
Description	Centrosomes are the major microtubule-organising centers in animals and play fundamental roles in many cellular processes. Understanding how their composition varies across diverse cell types and how it is altered in disease aremajor unresolved questions, yet currently available centrosome isolation protocols are cumbersome, time-consuming and lack scalability. Here, we report the development of centrosome affinity capture (CAPture)-mass spectrometry (MS), a powerful one-step method to obtain high-resolution centrosome proteomes from untransformed and primary cell lines. Utilising a synthetic peptide derived from CCDC61 protein, CAPture specifically isolates intact centrosomes. Importantly, as a bead-based affinity method, it enables rapid sample processing and multiplexing unlike conventional approaches. Our study demonstrates the power of CAPture-MS to elucidate cell type dependent heterogeneity in centrosome composition, dissect hierarchical interactions and identify novel components. Overall, CAPture-MS represents a transformative tool to unveil temporal, regulatory, cell type- and tissue-specific changes in centrosome proteomes in health and disease.
HostingRepository	PRIDE
AnnounceDate	2023-09-18
AnnouncementXML	Submission_2023-09-18_13:21:43.635.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	RanjaniGanji
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-19 05:22:15	ID requested
⏵ 1	2023-09-18 13:21:43	announced

Dataset with its publication pending

submitter keyword: appendages, centriole, proteome,Centrosome

JamesAult
contact affiliation	Mass Spectrometry Research Facility Manager
contact email	J.R.Ault@leeds.ac.uk
lab head
RanjaniGanji
contact affiliation	Univeristy of Leeds
contact email	ranjani.ganji@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043906
     1. Label: PRIDE project
     2. Name: Proteomic profiling of centrosomes across multiple cell and tissue types by a new affinity capture method