PXD043887

PXD043887 is an original dataset announced via ProteomeXchange.

Title	DJ-1 protects cells by catalyzing the hydrolysis of cyclic 3-phosphoglyceric anhydride - a highly reactive glycolytic byproduct
Description	Mutations in the human protein DJ-1/PARK7 are found to be involved in familial Parkinson’s disease. However, the molecular mechanism of the protein remains unclear. Lately, DJ-1 was found to prevent the acylation of amino groups in proteins. The process of acylation requires a cyclic 3-phosphoglyceric anhydride (cPGA). The cPGA has been shown to be an intermediate product of 1,3-biphosphoglycerate and to be destroyed by DJ-1’s hydrolase activity. In our study, we synthesized and characterized cPGA. Furthermore, we demonstrated an endogenous catalytic hydrolase activity of DJ-1 towards cPGA in crude protein extracts of human HCT116 cells. The addition of synthesized cPGA to DJ-1 knockout HCT116 cytoplasmic cell lysate caused a dramatic increase in phosphoglycerate-modified proteins. The submitted results contain proteomic analysis of phosphoglycerate modifications on lysine residues in wild type and DJ-1 knockout cytoplasmic proteins of HCT116 cells when cPGA was added. Our study confirms that DJ-1 prevents the formation of phosphoglycerate modifications on amino groups of proteins.
HostingRepository	iProX
AnnounceDate	2023-07-19
AnnouncementXML	Submission_2024-03-10_20:30:56.514.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aizhan Akhmadi
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	N6-(3-phosphoglyceryl)-L-lysine
Instrument	Bruker Daltonics instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-19 00:00:44	ID requested
1	2023-07-19 00:00:51	announced
⏵ 2	2024-03-10 20:30:57	announced	2024-03-11: Update information.

Akhmadi A, Yeskendir A, Dey N, Mussakhmetov A, Shatkenova Z, Kulyyassov A, Andreeva A, Utepbergenov D, DJ-1 protects proteins from acylation by catalyzing the hydrolysis of highly reactive cyclic 3-phosphoglyceric anhydride. Nat Commun, 15(1):2004(2024) [pubmed]

submitter keyword: Parkinson’s disease, DJ-1, PARK7, glycolysis, cyclic acylphosphate, cyclic phosphoglyceric anhydride, metabolite damage

Darkhan Utepbergenov
contact affiliation	Nazarbayev University
contact email	darkhan.utepbergenov@nu.edu.kz
lab head
Aizhan Akhmadi
contact affiliation	Nazarbayev University
contact email	aizhan.tkirova@nu.edu.kz
dataset submitter

iProX dataset URI