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PXD043882

PXD043882 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMatrix-Augmented Pooling Strategy for High-Throughput Target Deconvolution Reveals Cell Type Off-targets and Responses
DescriptionTarget deconvolution is a crucial but costly and time-consuming task that hinder large-scale profiling for drug discovery. We present a matrix-augmented pooling strategy (MAPS) which mixes multiple drugs into samples with optimized permutation and delineates targets of each drug simultaneously with mathematical processing. We validated this strategy with thermal proteome profiling (TPP) testing of 15 drugs concurrently, increasing experimental throughput by 60x while maintaining high sensitivity and specificity. Benefiting from the lower cost and higher throughput of MAPS, we perform target deconvolution of the 15 drugs across 5 cell lines. Our profiling revealed drug-target interactions can differ vastly in targets and binding affinity across cell lines. We further validated BRAF and CSNK2A are the potential off-targets of bafetinib and abemaciclib, respectively. This work presents the largest target profiling of structurally diverse drugs across multiple cell lines to date.
HostingRepositoryiProX
AnnounceDate2023-07-19
AnnouncementXMLSubmission_2023-07-18_21:34:23.295.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHongchao Ji
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListTMT6plex reporter fragment
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-18 21:33:05ID requested
12023-07-18 21:34:23announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: CETSA, Target Deconvolution, High-Throughput, Pooling Strategy
Contact List
Chris Tan
contact affiliationSouthern University of Science and Technology
contact emailchris.tan.sh@gmail.com
lab head
Hongchao Ji
contact affiliationSouthern University of Science and Technology
contact emailji.hongchao@foxmail.com
dataset submitter
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iProX dataset URI