PXD043857
PXD043857 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The quantitative proteomic analysis of Pseudomonas aeruginosa treated with or without Staphylococcus aureus-derived extracellular vesicles (SaEVs) |
| Description | Pseudomonas aeruginosa was prepared and adjusted to an initial bacterial number of OD600 = 1.0 in Tryptic soy broth with or without 5 μg/mL SaEVs. After incubation at 37°C for 24 h, the bacterial cells were collected by centrifugation at 1,100 x g, 4°C for 15 min, followed by washing twice with phosphate-buffered saline (PBS). The bacterial cells were lysed with 2% sodium dodecyl sulfate/ 7M urea, and equal amounts of protein from each sample were precipitated with acetone. The proteins were denatured with 50% trifluoroethanol and reduced using 4 mM dithiothreitol. Free cysteine residues were alkylated prior to trypsinization, and the desalted peptides were then separated using liquid chromatography. The mass spectrometer was operated in information-dependent acquisition mode. Acquired spectra were searched against the proteins of P. aeruginosa strain ATCC 15692. Differential protein expression was analyzed to determine the relative abundances of proteins in SaEV-treated P. aeruginosa compared to non-treated P. aeruginosa. |
| HostingRepository | jPOST |
| AnnounceDate | 2024-01-30 |
| AnnouncementXML | Submission_2024-01-29_17:59:47.532.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yota Tatara |
| SpeciesList | scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine |
| Instrument | TripleTOF 6600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-07-17 19:20:24 | ID requested | |
| ⏵ 1 | 2024-01-29 17:59:48 | announced |
Publication List
| Subsomwong P, Teng W, Ishiai T, Narita K, Sukchawalit R, Nakane A, Asano K, Extracellular vesicles from Staphylococcus aureus promote the pathogenicity of Pseudomonas aeruginosa. Microbiol Res, 281():127612(2024) [pubmed] |
Keyword List
| submitter keyword: Pseudomonas aeruginosa, Staphylococcus aureus, extracellular vesicles, SWATH |
Contact List
| Krisana Asano | |
|---|---|
| lab head | |
| Yota Tatara | |
| contact affiliation | Hirosaki University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002252/ |
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