PXD043854

PXD043854 is an original dataset announced via ProteomeXchange.

Title	The proteins identified from Staphylococcus aureus-derived extracellular vesicles (SaEVs)
Description	Staphylococcus aureus strain ATCC 1718 was inoculated at 0.5% in 400 mL of Brain Heart Infusion broth for 8 h under aerobic conditions. The supernatant was collected by centrifugation twice at 5,000 x g, 4°C for 20 min, filtrated through a 0.45 µm filter to remove all remaining bacterial cells. S. aureus-derived extracellular vesicles (SaEVs) in culture supernatant were precipitated by ultracentrifugation at 100,000 x g, 4°C for 90 min using a Himac CP80WX Preparative Ultracentrifuge (HITACHI, Tokyo, Japan). After washing with ice-cold PBS, the pellet was suspended and applied to discontinuous iodixanol gradient ultracentrifugation. Layers of 40%, 20%, 10%, and 5% iodixanol in 0.25 M sucrose/10 mM Tris, pH 7.5 were prepared using OptiPrep™ [60% (w/v) iodixanol; Sigma Aldrich, St. Louis, MO]. After centrifugation at 100,000 x g, 4°C for 16 h, the substances in all six fractions (Fr1-Fr6) were collected, diluted in PBS, and precipitated by ultracentrifugation at 100,000 × g, 4°C for 2 h. The pellets were then washed and resuspended in an appropriate volume of ice-cold PBS.
HostingRepository	jPOST
AnnounceDate	2024-01-30
AnnouncementXML	Submission_2024-01-29_18:00:36.179.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yota Tatara
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine; phosphorylated L-histidine; O-phospho-L-serine
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-17 18:50:41	ID requested
⏵ 1	2024-01-29 18:00:37	announced

Subsomwong P, Teng W, Ishiai T, Narita K, Sukchawalit R, Nakane A, Asano K, Extracellular vesicles from Staphylococcus aureus promote the pathogenicity of Pseudomonas aeruginosa. Microbiol Res, 281():127612(2024) [pubmed]

submitter keyword: Staphylococcus aureus, extracellular vesicles, proteome

Krisana Asano
lab head
Yota Tatara
contact affiliation	Hirosaki University
dataset submitter

jPOST dataset URI

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