PXD043853

PXD043853 is an original dataset announced via ProteomeXchange.

Title	The glycolytic signaling metabolite fructose-1,6-bisphosphate shapes cell adhesion and protrusion
Description	Cellular adhesion to the extracellular matrix is essential for morphogenesis, tissue integrity and survival signaling. The best understood adhesion structures are focal adhesions. Inspite of their importance, our knowledge of upstream regulatory factors that integrate focal adhesion assembly with other core cellular processes has remained scarce. To identify potential upstream factors, we have conducted a genome-wide screen for focal adhesion regulators. In this screen depletion of the glycolytic enzyme aldolase A which degrades fructose-1,6-bisphosphate caused an increase in focal adhesion number and cell size. Following up on this finding, we show here that fructose-1,6-bisphosphate is a crucial signaling metabolite transmitting information about the metabolic cell state for the adaptation of cell adhesion and protrusion. At the molecular level, fructose-1,6-bisphosphate relieves RCC2-mediated inhibition of Rac1 by direct binding to RCC2. The increase in Rac1 activity leads to reorganization of the actin cytoskeleton, an increase in focal adhesion assembly and elevated protrusive activity.
HostingRepository	PRIDE
AnnounceDate	2026-02-03
AnnouncementXML	Submission_2026-02-03_06:34:47.945.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilaria Piazza
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-17 15:16:01	ID requested
⏵ 1	2026-02-03 06:34:48	announced

Dataset with its publication pending

submitter keyword: cytoskeleton,metabolism

Ilaria Piazza
contact affiliation	MDC Berlin
contact email	ilaria.piazza@mdc-berlin.de
lab head
Ilaria Piazza
contact affiliation	MDC Berlin, BIMSB Berlin
contact email	ilaria.piazza@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD043853

PRIDE project URI

• PRIDE
  1. PXD043853
     1. Label: PRIDE project
     2. Name: The glycolytic signaling metabolite fructose-1,6-bisphosphate shapes cell adhesion and protrusion