PXD043840 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of Novel Scaffold Proteins for Improved Endogenous Engineering of Extracellular Vesicles |
Description | Extracellular vesicles (EVs) are gaining ground as next-generation drug delivery modalities. Genetic fusion of the protein of interest to a scaffold protein with high EV-sorting ability represents a robust cargo loading strategy. In this study 244 candidate scaffold proteins were studied, resulting in the identification of 24 proteins with conserved EV-sorting abilities across five types of producer cells. TSPAN2 and TSPAN3 were detected as the lead candidates for cargo loading, outperforming the well-known CD63 scaffold. EVs from human embryonic kidney epithelial (HEK-293T) cells, either wild type or overexpressing TSPAN2, TSPAN3 or CD63, were analyzed with label-free top-down proteomics to understand the effect of EV-engineering on protein signatures. The proteomics findings demonstrated that TSPAN2/TSPAN3-based engineering gives rise to EV subpopulations distinct from CD63. The discovery of these novel scaffolds provides a new platform for EV-based therapies. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:00:36.342.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Helena Sork |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-07-17 06:33:16 | ID requested | |
1 | 2023-08-18 05:08:34 | announced | |
2 | 2023-11-14 07:00:28 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 06:00:36 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: extracellular vesicles, tetraspanin, endogenous loading,exosome |
Contact List
Samir EL Andaloussi |
contact affiliation | Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden |
contact email | samir.el-andaloussi@ki.se |
lab head | |
Helena Sork |
contact affiliation | University of Tartu |
contact email | helena.sork@ut.ee |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD043840 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043840
- Label: PRIDE project
- Name: Identification of Novel Scaffold Proteins for Improved Endogenous Engineering of Extracellular Vesicles