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PXD043840

PXD043840 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of Novel Scaffold Proteins for Improved Endogenous Engineering of Extracellular Vesicles
DescriptionExtracellular vesicles (EVs) are gaining ground as next-generation drug delivery modalities. Genetic fusion of the protein of interest to a scaffold protein with high EV-sorting ability represents a robust cargo loading strategy. In this study 244 candidate scaffold proteins were studied, resulting in the identification of 24 proteins with conserved EV-sorting abilities across five types of producer cells. TSPAN2 and TSPAN3 were detected as the lead candidates for cargo loading, outperforming the well-known CD63 scaffold. EVs from human embryonic kidney epithelial (HEK-293T) cells, either wild type or overexpressing TSPAN2, TSPAN3 or CD63, were analyzed with label-free top-down proteomics to understand the effect of EV-engineering on protein signatures. The proteomics findings demonstrated that TSPAN2/TSPAN3-based engineering gives rise to EV subpopulations distinct from CD63. The discovery of these novel scaffolds provides a new platform for EV-based therapies.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:00:36.342.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHelena Sork
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-17 06:33:16ID requested
12023-08-18 05:08:34announced
22023-11-14 07:00:28announced2023-11-14: Updated project metadata.
32024-10-22 06:00:36announced2024-10-22: Updated project metadata.
Publication List
10.1038/S41467-023-40453-0;
Keyword List
submitter keyword: extracellular vesicles, tetraspanin, endogenous loading,exosome
Contact List
Samir EL Andaloussi
contact affiliationDivision of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden
contact emailsamir.el-andaloussi@ki.se
lab head
Helena Sork
contact affiliationUniversity of Tartu
contact emailhelena.sork@ut.ee
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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