PXD043840

PXD043840 is an original dataset announced via ProteomeXchange.

Title	Identification of Novel Scaffold Proteins for Improved Endogenous Engineering of Extracellular Vesicles
Description	Extracellular vesicles (EVs) are gaining ground as next-generation drug delivery modalities. Genetic fusion of the protein of interest to a scaffold protein with high EV-sorting ability represents a robust cargo loading strategy. In this study 244 candidate scaffold proteins were studied, resulting in the identification of 24 proteins with conserved EV-sorting abilities across five types of producer cells. TSPAN2 and TSPAN3 were detected as the lead candidates for cargo loading, outperforming the well-known CD63 scaffold. EVs from human embryonic kidney epithelial (HEK-293T) cells, either wild type or overexpressing TSPAN2, TSPAN3 or CD63, were analyzed with label-free top-down proteomics to understand the effect of EV-engineering on protein signatures. The proteomics findings demonstrated that TSPAN2/TSPAN3-based engineering gives rise to EV subpopulations distinct from CD63. The discovery of these novel scaffolds provides a new platform for EV-based therapies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:00:36.342.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Helena Sork
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-17 06:33:16	ID requested
1	2023-08-18 05:08:34	announced
2	2023-11-14 07:00:28	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:00:36	announced	2024-10-22: Updated project metadata.

10.1038/S41467-023-40453-0;

submitter keyword: extracellular vesicles, tetraspanin, endogenous loading,exosome

Samir EL Andaloussi
contact affiliation	Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden
contact email	samir.el-andaloussi@ki.se
lab head
Helena Sork
contact affiliation	University of Tartu
contact email	helena.sork@ut.ee
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD043840

PRIDE project URI

• PRIDE
  1. PXD043840
     1. Label: PRIDE project
     2. Name: Identification of Novel Scaffold Proteins for Improved Endogenous Engineering of Extracellular Vesicles