PXD043814

PXD043814 is an original dataset announced via ProteomeXchange.

Title	Effect of furoxans on smooth muscle cell proliferation by SILAC experiments
Description	Physiologically, smooth muscle cells (SMCs) and nitric oxide (NO) produced by endothelial cells strictly cooperate to maintain vascular homeostasis. In atherosclerosis, this equilibrium is altered, therefore molecules, able to provide exogenous NO and inhibit SMC proliferation, may represent valuable anti-atherosclerotic agents. Searching for dual anti-proliferative and NO-donor molecules, we found that furoxans significantly decreased SMC proliferation in vitro, albeit with different potencies. We therefore assessed whether this property is dependent on their thiol-induced ring opening. Indeed, while furazans (analogues unable to release NO) are not effective, furoxans’ inhibitory potency parallels with the electron-attractor capacity of the group in 3 of the ring, making this effect tunable. To demonstrate whether their specific block on G1-S phase could be NO-dependent, we supplemented SMCs with furoxans and inhibitors of GMP- and/or of the polyamines pathway, which regulate NO-induced SMC proliferation, but they failed in preventing the anti-proliferative effect. To find the real mechanism of this property, we performed a proteomics study, using a SILAC approach, revealing eleven cellular proteins (with SUMO1 being central) statistically deregulated by furoxans and involved in cell homeostasis/proliferation. Altogether, thanks to their dual effect and pharmacological flexibility, furoxans may be evaluated in future as anti-atherosclerotic molecules.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:59:15.078.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043814
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Annapaola Andolfo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-16 03:43:17	ID requested
1	2023-07-31 07:39:26	announced
2	2023-11-14 06:50:27	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:59:15	announced	2024-10-22: Updated project metadata.

10.3390/MOLECULES28155724;

10.6019/PXD043814;

submitter keyword: furoxans, nitric oxide,Atherosclerosis, SILAC, smooth muscle cell proliferation

Lorenzo Arnaboldi
contact affiliation	Università degli Studi di Milano Department of Pharmacological and Biomolecular Sciences“Rodolfo Paoletti” Via Balzaretti, 9 20133 Milan, Italy
contact email	lorenzo.arnaboldi@unimi.it
lab head
Annapaola Andolfo
contact affiliation	Ospedale San Raffaele
contact email	andolfo.annapaola@hsr.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD043814

PRIDE project URI

• PRIDE
  1. PXD043814
     1. Label: PRIDE project
     2. Name: Effect of furoxans on smooth muscle cell proliferation by SILAC experiments