PXD043788

PXD043788 is an original dataset announced via ProteomeXchange.

Title	Alanine Scanning-PRISMA of GATAD1 peptide
Description	To explore the role of individual amino acids in the binding of GATAD1 peptide (position 95-109) to 14-3-3 proteins, we conducted an alanine scanning experiment. Each amino acid surrounding the phosphorylation site within the peptide was systematically mutated to alanine, except for the original alanine residues, which were mutated to glycine. These mutant peptides were immobilized on a cellulose membrane and employed to capture proteins from Hek293T lysate through pulldown assays.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:30:01.414.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Trendelina Rrustemi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-14 05:57:34	ID requested
1	2024-02-15 07:00:36	announced
⏵ 2	2024-10-22 06:30:01	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: pull-down,Alanine scanning, 14-3-3, GATAD1

Matthias Selbach
contact affiliation	Max Delbrueck Center for Molecular Medicine in the Helmholtz Association (MDC)
contact email	matthias.selbach@mdc-berlin.de
lab head
Trendelina Rrustemi
contact affiliation	Max-Delbrück Center for Molecular Medicine
contact email	trendelina.rrustemi@mdc-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043788
     1. Label: PRIDE project
     2. Name: Alanine Scanning-PRISMA of GATAD1 peptide