PXD043787

PXD043787 is an original dataset announced via ProteomeXchange.

Title	PRISMA screen on pathogenic mutations that destroy phosphorylation sites within intrinsically disordered regions
Description	Many of the disease-causing point mutations occur within structure-lacking intrinsically disordered regions (IDRs) of proteins. IDRs often contain short linear motifs (SLiMs) that are crucial for protein-protein interactions (PPIs) and are often subject to phosphorylation. Our approach involved immobilizing synthetic peptides representing mutated phosphorylation sites within the IDR regions onto cellulose membranes to capture interacting proteins from cellular extracts. This enabled simultaneous comparison of interaction partners between wild-type, phosphorylated, and mutated peptide forms, allowing functional assessment of individual mutations. We screened 36 disease-causing phosphorylation site mutations within IDRs, sourced from PTMVar database. The results revealed substantial differences between phosphorylated and mutated peptide interactomes, often due to disrupted phosphorylated SLiMs
HostingRepository	PRIDE
AnnounceDate	2024-02-15
AnnouncementXML	Submission_2024-02-15_08:22:58.344.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Trendelina Rrustemi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-14 05:26:31	ID requested
⏵ 1	2024-02-15 08:22:58	announced

Dataset with its publication pending

submitter keyword: pull-down, peptide, IDR, human-disease,PRISMA, phosphorylation

Matthias Selbach
contact affiliation	Max Delbrueck Center for Molecular Medicine in the Helmholtz Association
contact email	matthias.selbach@mdc-berlin.de
lab head
Trendelina Rrustemi
contact affiliation	Max-Delbrück Center for Molecular Medicine
contact email	trendelina.rrustemi@mdc-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043787
     1. Label: PRIDE project
     2. Name: PRISMA screen on pathogenic mutations that destroy phosphorylation sites within intrinsically disordered regions