PXD043778
PXD043778 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Overcoming the detrimental O-acylation in TMTpro labeling improves the proteome depth and quantification precision. |
Description | The advent of TMTpro reagents expands the sample multiplexing capacity and enables quantification of up to 18 samples in a multiplexed manner. Similar to classic TMT reagents, TMTpro reagents contain a tertiary amine group in their mole-cules, which markedly enhances their reactivity toward hydroxyl groups and thus results in overlabeling of serine, threo-nine and tyrosine residues. Overlabeling significantly compromises the proteome analysis in terms of sensitivity and precision. Here, we report a novel TMTpro labeling method that overcomes the detrimental overlabeling while delivering efficient labeling for amines. Additionally, our method is cost-effective as it requires only half the amount of TMTpro reagents recommended by the reagent manufacturer. We compared our method with the standard method (provided by the TMTpro manufacturer) in a deep-scale analysis of a yeast/human two-proteome model sample. Even at the depth of over 10000 proteins, our method detected 36.2% more unique peptides and 11.5% more protein groups than the standard method. Moreover, our method considerably improves the quantitative precision due to the reduced variability in label-ing and increased protein sequence coverage. This substantially enhanced the statistical power of our method for detect-ing differentially abundant proteins, providing an average of 16% more yeast proteins reaching statistical significance. |
HostingRepository | iProX |
AnnounceDate | 2023-12-19 |
AnnouncementXML | Submission_2024-04-27_22:39:40.188.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yan Cai |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-07-13 22:11:45 | ID requested | |
⏵ 1 | 2024-04-27 22:39:41 | announced |
Publication List
Cai Y, Chang C, Liao R, Overcoming the detrimental O-acylation in TMTpro labeling improves the proteome depth and quantification precision. Anal Chim Acta, 1304():342538(2024) [pubmed] |
Keyword List
submitter keyword: Proteomics, TMTpro, LC-MS/MS |
Contact List
Rijing Liao | |
---|---|
contact affiliation | Shanghai Institute of Precision Medicine, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine |
contact email | rjliao@shsmu.edu.cn |
lab head | |
Yan Cai | |
contact affiliation | Shanghai Ninth People ‘s Hospital |
contact email | caiyan0723@shsmu.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |