PXD043776
PXD043776 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | BCAT1 Confers Resistance to EGFR TKIs through α-KG-dependent Epigenetic Regulation of the Glycolysis Pathway in NSCLC |
Description | Epidermal growth factor receptor (EGFR) mutations are leading oncogenic drivers in non-small cell lung cancer (NSCLC). Many third-generation EGFR tyrosine kinase inhibitors (TKIs) have been developed to target EGFR T790M and activating mutations. Osimertinib (AZD9291) was the first approved drug and is now the standard-of-care therapy for untreated EGFR-mutated NSCLC. Our team previously identified ASK120067, for which we have submitted a new drug application (NDA) in China. Although third-generation EGFR TKIs exhibit favorable antitumor effects in NSCLC treatment, acquired resistance with largely unexplored mechanisms still limits their long-term efficacy. In this study, we aimed to investigate the molecular mechanisms underlying resistance to EGFR TKIs and identify new therapeutic strategies for the treatment of NSCLC. Methods: Elevated expression of BCAT1 in EGFR TKI-resistant lung cancer cells was identified using stable isotope labeling by amino acids in cell culture (SILAC)-based proteomics analysis and verified with Western blotting and RT‒qPCR. |
HostingRepository | iProX |
AnnounceDate | 2023-07-14 |
AnnouncementXML | Submission_2024-08-15_19:30:51.951.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jing Gao |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-07-13 19:49:36 | ID requested | |
⏵ 1 | 2024-08-15 19:30:52 | announced |
Publication List
Zhang T, Pan Z, Gao J, Wu Q, Bai G, Li Y, Tong L, Feng F, Lai M, Liu Y, Song P, Ning Y, Tang H, Luo W, Chen Y, Fang Y, Zhang H, Liu Q, Zhang Y, Wang H, Chen Z, Chen Y, Geng M, Ji H, Zhao G, Zhou H, Ding J, Xie H, Branched-chain amino acid transaminase 1 confers EGFR-TKI resistance through epigenetic glycolytic activation. Signal Transduct Target Ther, 9(1):216(2024) [pubmed] |
Keyword List
submitter keyword: EGFR TKI, Drug resistance, BCAT1, α-KG, Glycolysis |
Contact List
Hu Zhou | |
---|---|
contact affiliation | Shanghai Institute of Materia Medica, Chinese Academy of Sciences |
contact email | zhouhu@simm.ac.cn |
lab head | |
Jing Gao | |
contact affiliation | Shanghai Institute of Materia Medica, Chinese Academy of Sciences |
contact email | jinggao@simm.ac.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |