PXD043754

PXD043754 is an original dataset announced via ProteomeXchange.

Title	Human Histone Sulfated Tyrosine Identification
Description	Tyrosine sulfation is a common posttranslational modification in mammals. To date, it has been thought to be limited to secreted and transmembrane proteins, but little is known about tyrosine sulfation on nuclear proteins.To search for tyrosine sulfation on nuclear proteins, we purified the nucleus of HepG2 cells and searched for Ysulf using HPLC–MS/MS.We noted a peptide that is highly indicative of sulfation on the tyrosine 99 residue of histone H3(H3Y99sulf).We synthesized the peptides with the same primary sequence and modifications as were identified in the HPLC–MS/MS analysis: The synthesized H3Y99sulf peptides and their in vivo counterparts showed similar chromatographic and mass spectrum profiles in the same HPLC–MS/MS system . These results demonstrate that H3Y99sulf is an undocumented histone modification
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:09.104.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weixing Yu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	sulfated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-13 05:25:43	ID requested
1	2023-07-13 05:59:41	announced
⏵ 2	2023-11-14 09:02:10	announced	2023-11-14: Updated project metadata.

Yu W, Zhou R, Li N, Lei ZC, Guo D, Peng F, Li Y, Bai X, Feng S, Wang Y, He J, Yin S, Zeng X, He L, Gao Y, Li M, Guo YR, Liu K, Wang Y, Histone tyrosine sulfation by SULT1B1 regulates H4R3me2a and gene transcription. Nat Chem Biol, 19(7):855-864(2023) [pubmed]

submitter keyword: Human,LC-MS/MS,Histone Sulfation,Liver

Yugang Wang
contact affiliation	Huazhong University of Science and Technology
contact email	yugangw@hust.edu.cn
lab head
Weixing Yu
contact affiliation	Huazhong University of Science and Technology
contact email	moxing77@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD043754

PRIDE project URI

• PRIDE
  1. PXD043754
     1. Label: PRIDE project
     2. Name: Human Histone Sulfated Tyrosine Identification