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PXD043746

PXD043746 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDynorphin-sensing two-component systems ParRS and CprRS in Pseudomonas aeruginosa pathogenesis
DescriptionThe widespread presence of antibiotic-resistant bacteria in the environment has been recognized as an important emerging environmental contaminant. Hospital wards, as a special public indoor environment, are of great concern for the risks associated with this emerging environmental contaminant. Pseudomonas aeruginosa, a common nosocomial bacterium, is a contamination risk in the hospital environment due to its drug resistance and transmission of virulence factors. Notably, the antimicrobial peptide-sensing two-component system (TCS) ParRS and CprRS have been implicated in dynorphin-induced signaling, but the underlying Manuscript2 mechanism has remained elusive. In this study, we performed proteomic analysis to systematically investigate the contributions of ParRS and CprRS to P. aeruginosa pathogenesis and dynorphin-induced resistance to polymyxins. Additionally, we characterized the significance of the extracellular sensor domains of ParS and CprS in dynorphin perception. Furthermore, through structural biology, we identified additional TCS sensors with similar extracellular domain conformations, which also directly interacted with dynorphin in vitro. This suggests convergent evolution in different bacterial TCSs for host-derived synthetic peptide signal transmitting. Our findings establish a link between CAMPs resistance associated TCSs and virulence regulation of common nosocomial bacteria. This further illustrates the danger of this emerging contaminant for the environment and humans.
HostingRepositoryPRIDE
AnnounceDate2024-07-04
AnnouncementXMLSubmission_2024-07-03_19:19:05.531.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterhanzhong feng
SpeciesList scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-13 01:42:33ID requested
12024-07-03 19:19:05announced
Publication List
10.1016/J.JHAZMAT.2023.132512;
Keyword List
submitter keyword: Pseudomonas aeruginosa
ParRS
CprRS
resistance
dynorphin
Contact List
Yomngxing He
contact affiliationLanzhou University, Lanzhou, Gansu, China
contact emailheyx@lzu.edu.cn
lab head
hanzhong feng
contact affiliationLanzhou University
contact emailfenghzh19@lzu.edu.cn
dataset submitter
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Dataset FTP location
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