PXD043727

PXD043727 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of proteome of THP-1 macrophages incubated with Paracoccidioides brasiliensis yeast cells alive or heat-killed
Description	Paracoccidioides spp. is the etiological agent of Paracoccidioidomycosis (PCM), a systemicinfection with wide distribution in Latin America. Macrophages are very important cells during the response to infection by Paracoccidoides brasiliensis and understanding the interaction between the fungus and immune cells is very relevant for understanding the disease. In this study, we performed a proteomic analysis to assess the consequences of P. brasiliensis yeast infection on the THP-1 macrophage proteome and to verify whether there are differences between the proteome of cells infected with the live fungus. We identified 443 upregulated and 2247 downregulated proteins in macrophages infected with live P. brasiliensis yeasts, compared to uninfected macrophages. Proteins differentially expressed in cells infected are related to metabolism and energy production, protein synthesis and processing, transcription, cell cycle, DNA processing, cell signaling, oxidative stress, immune response, among other processes . Proteomic analysis revealed that P. brasiliensis, causes metabolic alterations in infected cells, drastically affecting energy production pathways. In addition, macrophages showed many upregulated, mostly downregulated, immune system proteins. Thus, the present work contributes to elucidate the changes that occur in immune cells in response to infection by P. brasiliensis and may help to better understand PCM.
HostingRepository	PRIDE
AnnounceDate	2023-10-23
AnnouncementXML	Submission_2023-10-23_11:15:39.216.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CeliaSoares
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; carbamoylated residue; monohydroxylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-12 12:20:46	ID requested
⏵ 1	2023-10-23 11:15:39	announced

Dataset with its publication pending

submitter keyword: P. brasiliensis

Proteomic analysis

Metabolic reprogramming

Immunometabolism

Celia Maria AlmeidaSoares
contact affiliation	Universidade Federal de Goias
contact email	cmasoares@gmail.com
lab head
CeliaSoares
contact affiliation	UFG
contact email	hplcmslbm@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043727
     1. Label: PRIDE project
     2. Name: Proteomic analysis of proteome of THP-1 macrophages incubated with Paracoccidioides brasiliensis yeast cells alive or heat-killed