PXD043724

PXD043724 is an original dataset announced via ProteomeXchange.

Title	Proteomic exploration of SSR42 and its impact on Staphylococcus aureus virulence
Description	Perhaps the most pivotal feature contributing to Staphylococcus aureus pathogenicity is the production of a wealth of virulence factors such as secreted toxins, adhesive proteins, and factors that aid in host immune evasion. The abundance and activity of these factors is tightly regulated by a multitude of effectors, including two-component systems, alternative sigma factors, DNA binding transcription factors, and regulatory RNAs. SSR42 is a ~1,200-nt long regulatory RNA that has been previously shown to be highly stable and abundant in stationary phase growth. To explore the role of SSR42 within the S. aureus cell, we created a full deletion mutant for this gene and performed mass spectrometry analysis on all protein fractions after 15h of growth. Following standardized bacterial growth and sample processing, peptides were analyzed using a hybrid Quadrupole-Orbitrap instrument and spectra were assigned with MaxQuant to the USA300 pan-proteome (UP000001939). When compared to wild-type, the SSR42 mutant exhibited profound changes in its proteomic landscape, suggesting that SSR42 has myriad regulatory targets across niches of function.
HostingRepository	PRIDE
AnnounceDate	2025-04-17
AnnouncementXML	Submission_2025-04-17_10:24:45.075.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emilee Mustor
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-12 08:59:53	ID requested
⏵ 1	2025-04-17 10:24:45	announced

10.1101/2024.07.11.603084;

submitter keyword: SSR42, bacteria, Orbitrap,Staphylococcus aureus, pathogenesis

Lindsey Neil Shaw
contact affiliation	Department of Molecular Biosciences Shaw Lab University of South Florida 4202 East Fowler Avenue, ISA2015 Tampa, FL 33620-5150 USA
contact email	shaw@usf.edu
lab head
Emilee Mustor
contact affiliation	University of South Florida
contact email	emileemustor@usf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD043724

PRIDE project URI

• PRIDE
  1. PXD043724
     1. Label: PRIDE project
     2. Name: Proteomic exploration of SSR42 and its impact on Staphylococcus aureus virulence