PXD043667
PXD043667 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | ProteasomeID: quantitative mapping of proteasome interactomes and substrates for in vitro and in vivo studies |
| Description | Proteasomes are essential molecular machines responsible for the degradation of proteins in eukaryotic cells. Altered proteasome activity has been linked to neurodegeneration, auto-immune disorders and cancer. Despite the relevance for human disease and drug development, no method currently exists to monitor proteasome composition and interactions in vivo in animal models. To fill this gap, we developed a strategy based on tagging of proteasomes with promiscuous biotin ligases and generated a new mouse model enabling the quantification of proteasome interactions by mass spectrometry. We show that biotin ligases can be incorporated in fully assembled proteasomes without negative impact on their activity. We demonstrate the utility of our method by identifying novel proteasome-interacting proteins, charting interactomes across mouse organs, and showing that proximity-labeling enables the identification of both endogenous and small molecule-induced proteasome substrates. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-07-05 |
| AnnouncementXML | Submission_2024-07-05_13:51:22.660.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Leibniz FLI Proteomics |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-07-11 00:32:10 | ID requested | |
| ⏵ 1 | 2024-07-05 13:51:23 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: proteasome, BioID, mass spectrometry, protein degradation, proximity labeling, mouse model |
Contact List
| Alessandro Ori | |
|---|---|
| contact affiliation | Leibniz Institute on Aging Fritz Lipmann Institute (FLI) |
| contact email | alessandro.ori@leibniz-fli.de |
| lab head | |
| Leibniz FLI Proteomics | |
| contact affiliation | Leibniz FLI |
| contact email | proteomics@leibniz-fli.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000092396/ |
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