PXD043660 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Deep Proteomic Compound Profiling with the Orbitrap Ascend Tribrid Mass Spectrometer Using Tandem Mass Tags and Real-Time Search |
Description | We evaluated the performance of the Orbitrap Ascend in comparison to the Orbitrap Eclipse in RTS-SPS-MS3-based analysis of human proteomes using fractionated and unfractionated samples labeled with TMT and TMTpro reagents. We did this comparison in the context of different sample input amounts; we analyzed differences in quantitative precision; we compared the effectiveness in identifying protein abundance changes induced by treatment with covalent inhibitors of PIN1. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:05:53.062.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Steven Shuken |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-07-10 15:02:06 | ID requested | |
1 | 2023-10-13 07:44:22 | announced | |
2 | 2023-11-14 09:06:05 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 06:05:54 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1021/acs.analchem.3c01701; |
Shuken SR, McAlister GC, Barshop WD, Canterbury JD, Bergen D, Huang J, Huguet R, Paulo JA, Zabrouskov V, Gygi SP, Yu Q, Deep Proteomic Compound Profiling with the Orbitrap Ascend Tribrid Mass Spectrometer Using Tandem Mass Tags and Real-Time Search. Anal Chem, 95(41):15180-15188(2023) [pubmed] |
Keyword List
submitter keyword: drug discovery, proteomics, mass spectrometry,TMT |
Contact List
Qing Yu |
contact affiliation | Department of Cell Biology, Harvard Medical School |
contact email | qing_yu2@hms.harvard.edu |
lab head | |
Steven Shuken |
contact affiliation | Department of Cell Biology, Harvard Medical School |
contact email | steven_shuken@hms.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD043660 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043660
- Label: PRIDE project
- Name: Deep Proteomic Compound Profiling with the Orbitrap Ascend Tribrid Mass Spectrometer Using Tandem Mass Tags and Real-Time Search