PXD043660

PXD043660 is an original dataset announced via ProteomeXchange.

Title	Deep Proteomic Compound Profiling with the Orbitrap Ascend Tribrid Mass Spectrometer Using Tandem Mass Tags and Real-Time Search
Description	We evaluated the performance of the Orbitrap Ascend in comparison to the Orbitrap Eclipse in RTS-SPS-MS3-based analysis of human proteomes using fractionated and unfractionated samples labeled with TMT and TMTpro reagents. We did this comparison in the context of different sample input amounts; we analyzed differences in quantitative precision; we compared the effectiveness in identifying protein abundance changes induced by treatment with covalent inhibitors of PIN1.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:05:53.062.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Shuken
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-10 15:02:06	ID requested
1	2023-10-13 07:44:22	announced
2	2023-11-14 09:06:05	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:05:54	announced	2024-10-22: Updated project metadata.

10.1021/acs.analchem.3c01701;

Shuken SR, McAlister GC, Barshop WD, Canterbury JD, Bergen D, Huang J, Huguet R, Paulo JA, Zabrouskov V, Gygi SP, Yu Q, Deep Proteomic Compound Profiling with the Orbitrap Ascend Tribrid Mass Spectrometer Using Tandem Mass Tags and Real-Time Search. Anal Chem, 95(41):15180-15188(2023) [pubmed]

submitter keyword: drug discovery, proteomics, mass spectrometry,TMT

Qing Yu
contact affiliation	Department of Cell Biology, Harvard Medical School
contact email	qing_yu2@hms.harvard.edu
lab head
Steven Shuken
contact affiliation	Department of Cell Biology, Harvard Medical School
contact email	steven_shuken@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD043660

PRIDE project URI

• PRIDE
  1. PXD043660
     1. Label: PRIDE project
     2. Name: Deep Proteomic Compound Profiling with the Orbitrap Ascend Tribrid Mass Spectrometer Using Tandem Mass Tags and Real-Time Search