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PXD043660

PXD043660 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeep Proteomic Compound Profiling with the Orbitrap Ascend Tribrid Mass Spectrometer Using Tandem Mass Tags and Real-Time Search
DescriptionWe evaluated the performance of the Orbitrap Ascend in comparison to the Orbitrap Eclipse in RTS-SPS-MS3-based analysis of human proteomes using fractionated and unfractionated samples labeled with TMT and TMTpro reagents. We did this comparison in the context of different sample input amounts; we analyzed differences in quantitative precision; we compared the effectiveness in identifying protein abundance changes induced by treatment with covalent inhibitors of PIN1.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:05:53.062.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSteven Shuken
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-10 15:02:06ID requested
12023-10-13 07:44:22announced
22023-11-14 09:06:05announced2023-11-14: Updated project metadata.
32024-10-22 06:05:54announced2024-10-22: Updated project metadata.
Publication List
10.1021/acs.analchem.3c01701;
Shuken SR, McAlister GC, Barshop WD, Canterbury JD, Bergen D, Huang J, Huguet R, Paulo JA, Zabrouskov V, Gygi SP, Yu Q, Deep Proteomic Compound Profiling with the Orbitrap Ascend Tribrid Mass Spectrometer Using Tandem Mass Tags and Real-Time Search. Anal Chem, 95(41):15180-15188(2023) [pubmed]
Keyword List
submitter keyword: drug discovery, proteomics, mass spectrometry,TMT
Contact List
Qing Yu
contact affiliationDepartment of Cell Biology, Harvard Medical School
contact emailqing_yu2@hms.harvard.edu
lab head
Steven Shuken
contact affiliationDepartment of Cell Biology, Harvard Medical School
contact emailsteven_shuken@hms.harvard.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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