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PXD043651

PXD043651 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titleo Differential temporal release and lipoprotein loading in B. thetaiotaomicron bacterial extracellular vesicles
Descriptiono Bacterial extracellular vesicles (BEVs) contribute to stress responses, quorum sensing, biofilm formation, and interspecies and interkingdom communication. However, the factors that regulate their release and heterogeneity are not well understood. We set out to investigate these factors in the common gut commensal Bacteroides thetaiotaomicron by studying BEV release throughout their growth cycle. Utilising a range of methods, we demonstrate that vesicles released at different stages of growth have significantly different composition, with early vesicles enriched in specifically released outer membrane vesicles (OMVs) containing a larger proportion of lipoproteins, while late phase BEVs primarily contain lytic vesicles with enrichment of cytoplasmic proteins. Furthermore, we demonstrate that lipoproteins containing a negatively charged signal peptide are preferentially incorporated in OMVs. We use this observation to predict all Bacteroides thetaiotaomicron OMV enriched lipoproteins and analyse their function. Overall, our findings highlight the need to understand media composition and BEV release dynamics prior to functional characterisation and define the theoretical functional capacity of Bacteroides thetaiotaomicron OMVs.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:24:37.503.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD043651
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterGerhard Saalbach
SpeciesList scientific name: Bacteroides thetaiotaomicron; NCBI TaxID: 818;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-10 10:13:45ID requested
12024-01-22 11:05:58announced
22024-10-22 06:24:37announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD043651;
Keyword List
submitter keyword: o Outer membrane vesicles
OMV
Bacterial extracellular vesicles
BEV
Bacteroides
Contact List
Simon Carding
contact affiliationQuadram Institute Rosalind Franklin Road, Norwich Research Park, Norwich, NR4 7UQ, United Kingdom
contact emailSimon.Carding@quadram.ac.uk
lab head
Gerhard Saalbach
contact affiliationBiological Chemistry
contact emailgerhard.saalbach@jic.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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